Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/43523
Title: Hexokinase 2, Glycogen Synthase and Phosphorylase Play a Key Role in Muscle Glycogen Supercompensation
Author: Irimia, JM.
Rovira, J.
Nielsen, Jakob N.
Guerrero, Mario
Wojtaszewski, J.
Cussó Fresquet, M. Roser
Keywords: Glicogen
Glucòlisi
Múscul llis
Glycogen
Glycolysis
Smooth muscle
Issue Date: 31-Jul-2012
Publisher: Public Library of Science (PLoS)
Abstract: Background: Glycogen-depleting exercise can lead to supercompensation of muscle glycogen stores, but the biochemical mechanisms of this phenomenon are still not completely understood. Methods: Using chronic low-frequency stimulation (CLFS) as an exercise model, the tibialis anterior muscle of rabbits was stimulated for either 1 or 24 hours, inducing a reduction in glycogen of 90% and 50% respectively. Glycogen recovery was subsequently monitored during 24 hours of rest. Results: In muscles stimulated for 1 hour, glycogen recovered basal levels during the rest period. However, in those stimulated for 24 hours, glycogen was supercompensated and its levels remained 50% higher than basal levels after 6 hours of rest, although the newly synthesized glycogen had fewer branches. This increase in glycogen correlated with an increase in hexokinase-2 expression and activity, a reduction in the glycogen phosphorylase activity ratio and an increase in the glycogen synthase activity ratio, due to dephosphorylation of site 3a, even in the presence of elevated glycogen stores. During supercompensation there was also an increase in 59-AMP-activated protein kinase phosphorylation, correlating with a stable reduction in ATP and total purine nucleotide levels. Conclusions: Glycogen supercompensation requires a coordinated chain of events at two levels in the context of decreased cell energy balance: First, an increase in the glucose phosphorylation capacity of the muscle and secondly, control of the enzymes directly involved in the synthesis and degradation of the glycogen molecule. However, supercompensated glycogen has fewer branches.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0042453
It is part of: PLoS One, 2012, vol. 7, num. 7, p. e42453
Related resource: http://dx.doi.org/10.1371/journal.pone.0042453
URI: http://hdl.handle.net/2445/43523
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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