Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/43604
Title: Most of the Abundant Protein Fractions of Embryonic Cerebrospinal Fluid are Produced Out of the Brain Anlagen
Author: Parvas, Maryam
Rius Viladomiu, Marc
Bueno i Torrens, David, 1965-
Keywords: Sistema nerviós central
Cervell
Neurobiologia
Central nervous system
Brain
Neurobiology
Issue Date: 2008
Publisher: Bentham Open
Abstract: The microenvironment of the central nervous system is important for neuronal function and development. During the early stages of embryo development the cephalic vesicles are filled by embryonic cerebrospinal fluid, a complex fluid containing different protein fractions, which contributes to the regulation of the survival, proliferation and neurogenesis of neuroectodermal stem cells. The protein content of embryonic cerebrospinal fluid from chick and rat embryos at the start of neurogenesis has already been determined. Most of the identified gene products are thought to be involved in the regulation of developmental processes during embryogenesis. However, due to the crucial roles played by embryonic cerebrospinal fluid during brain development, the embryological origin of the gene products it contains remains an intriguing question. According to the literature most of these products are synthesised in embryonic tissues other than the neuroepithelium. In this study we examined the embryological origin of the most abundant embryonic cerebrospinal fluid protein fractions by means of slot-blot analysis and by using several different embryonic and extraembryonic protein extracts, immunodetected with polyclonal antibodies. This first attempt to elucidate their origin is not based on the proteins identified by proteomic methods, but rather on crude protein fractions detected by SDS-PAGE analysis and to which polyclonal antibodies were specifically generated. Despite some of the limitations of this study, i.e. that one protein fraction may contain more than one gene product, and that a specific gene product may be contained in different protein fractions depending on post-translational modifications, our results show that most of the analysed protein fractions are not produced by the cephalic neuroectoderm but are rather stored in the egg reservoir; furthermore, few are produced by embryo tissues, thus indicating that they must be transported from their production or storage sites to the cephalic cavities, most probably via embryonic serum. These results raise the question as to whether the transfer of proteins from these two embryo compartments is regulated at this early developmental stage.
Note: Reproducció del document publicat a: http://dx.doi.org/10.2174/1875039700801010001
It is part of: Open Proteomics Journal, 2008, vol. 1, p. 1-4
Related resource: http://dx.doi.org/10.2174/1875039700801010001
URI: http://hdl.handle.net/2445/43604
ISSN: 1875-0397
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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