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http://hdl.handle.net/2445/43606
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DC Field | Value | Language |
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dc.contributor.author | Angulo, Sandra | - |
dc.contributor.author | Morales Muñoz, Albert | - |
dc.contributor.author | Danese, Silvio | - |
dc.contributor.author | Llacuna Durán, Laura | - |
dc.contributor.author | Masamunt, Maria Carme | - |
dc.contributor.author | Pultz, Nicole | - |
dc.contributor.author | Cifone, Maria Grazia | - |
dc.contributor.author | De Simone, Claudio | - |
dc.contributor.author | Delgado Rivilla, Salvadora | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.contributor.author | Panés Díaz, Julià | - |
dc.contributor.author | Donskey, Curtis | - |
dc.contributor.author | Fernández-Checa Torres, José Carlos | - |
dc.contributor.author | Fiocchi, Claudio | - |
dc.contributor.author | Sans i Cuffí, Miquel | - |
dc.date.accessioned | 2013-05-21T11:55:52Z | - |
dc.date.available | 2013-05-21T11:55:52Z | - |
dc.date.issued | 2011-03-09 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/43606 | - |
dc.description.abstract | Background: Probiotics appear to be beneficial in inflammatory bowel disease, but their mechanism of action is incompletely understood. We investigated whether probiotic-derived sphingomyelinase mediates this beneficial effect. Methodology/Principal Findings: Neutral sphingomyelinase (NSMase) activity was measured in sonicates of the probiotic L.brevis (LB)and S. thermophilus (ST) and the non-probiotic E. coli EC) and E. faecalis (EF). Lamina propria mononuclear cells (LPMC) were obtained from patients with Crohn"s disease (CD) and Ulcerative Colitis (UC), and peripheral blood mononuclear cells (PBMC) from healthy volunteers, analysing LPMC and PBMC apoptosis susceptibility, reactive oxygen species (ROS) generation and JNK activation. In some experiments, sonicates were preincubated with GSH or GW4869, a specific NSMase inhibitor. NSMase activity of LB and ST was 10-fold that of EC and EF sonicates. LB and ST sonicates induced significantly more apoptosis of CD and UC than control LPMC, whereas EC and EF sonicates failed to induce apoptosis. Pre-stimulation with anti-CD3/CD28 induced a significant and time-dependent increase in LB-induced apoptosis of LPMC and PBMC. Exposure to LB sonicates resulted in JNK activation and ROS production by LPMC. NSMase activity of LB sonicates was completely abrogated by GW4869, causing a dose-dependent reduction of LB -induced poptosis. LB and ST selectively induced immune cell apoptosis, an effect dependent on the degree of cell activation and mediated by bacterial NSMase. Conclusions: These results suggest that induction of immune cell apoptosis is a mechanism of action of some probiotics and that NSMase-mediated ceramide generation contributes to the therapeutic effects of probiotics. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0016953 | - |
dc.relation.ispartof | PLoS One, 2011, vol. 6, num. 3, p. e16953 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0016953 | - |
dc.rights | cc-by (c) Angulo, S. et al., 2011 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Malalties inflamatòries intestinals | - |
dc.subject.classification | Probiòtics | - |
dc.subject.other | Inflammatory bowel diseases | - |
dc.subject.other | Probiotics | - |
dc.title | Probiotic Sonicates Selectively Induce Mucosal Immune Cells Apoptosis Through Ceramide Generation Via Neutral Sphingolyelinase | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 593101 | - |
dc.date.updated | 2013-05-21T11:55:52Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 21408067 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
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593101.pdf | 1.27 MB | Adobe PDF | View/Open |
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