Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/43770
Title: The Rose Bengal test in human brucellosis: a neglected test for the diagnosis of a neglected disease
Author: Díaz, Ramón (Díaz García)
Casanova Rituerto, Aurora
Ariza Cardenal, Javier
Moriyón, Ignacio
Keywords: Brucel·losi
Malalties bacterianes
Diagnòstic microbiològic
Brucellosis
Bacterial diseases
Diagnostic microbiology
Issue Date: 2011
Publisher: Public Library of Science (PLoS)
Abstract: Brucellosis is a highly contagious zoonosis affecting livestock and human beings. The human disease lacks pathognomonic symptoms and laboratory tests are essential for its diagnosis. However, most tests are difficult to implement in the areas and countries were brucellosis is endemic. Here, we compared the simple and cheap Rose Bengal Test (RBT) with serum agglutination, Coombs, competitive ELISA, Brucellacapt, lateral flow immunochromatography for IgM and IgG detection and immunoprecipitation with Brucella proteins. We tested 208 sera from patients with brucellosis proved by bacteriological isolation, 20 contacts with no brucellosis, and 1559 sera of persons with no recent contact or brucellosis symptoms. RBT was highly sensitive in acute and long evolution brucellosis cases and this related to its ability to detect IgM, IgG and IgA, to the absence of prozones, and to the agglutinating activity of blocking IgA at the pH of the test. RBT was also highly specific in the sera of persons with no contact with Brucella. No test in this study outperformed RBT, and none was fully satisfactory in distinguishing contacts from infected patients. When modified to test serum dilutions, a diagnostic titer >4 in RBT resulted in 87.4% sensitivity (infected patients) and 100% specificity (contacts). We discuss the limitations of serological tests in the diagnosis of human brucellosis, particularly in the more chronic forms, and conclude that simplicity and affordability of RBT make it close to the ideal test for small and understaffed hospitals and laboratories.
Note: Reproducció del document publicat a: 10.1371/journal.pntd.0000950
It is part of: PLoS Neglected Tropical Diseases, 2011, vol. 5, num. 4, p. e950
Related resource: http://dx.doi.org/10.1371/journal.pntd.0000950
URI: http://hdl.handle.net/2445/43770
ISSN: 1935-2735
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)

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