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Title: Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration (in press)
Author: Chen, Zaozao
Lessey, Elizabeth
Berginski, Matthew E.
Cao, Li
Li, Jonathan
Trepat Guixer, Xavier
Itano, Michelle
Gómez, Shawn M.
Kapustina, Maryna
Huang, Cai
Burridge, Keith
Truskey, George
Jacobson, Ken
Keywords: Proteïnes quinases
Motilitat cel·lular
Protein kinases
Cell motility
Issue Date: Jan-2013
Publisher: Public Library of Science (PLoS)
Abstract: The issue of how contractility and adhesion are related to cell shape and migration pattern remains largely unresolved. In this paper we report that Gleevec (Imatinib), an Abl family kinase inhibitor, produces a profound change in the shape and migration of rat bladder tumor cells (NBTII) plated on collagen-coated substrates. Cells treated with Gleevec adopt a highly spread D-shape and migrate more rapidly with greater persistence. Accompanying this more spread state is an increase in integrin-mediated adhesion coupled with increases in the size and number of discrete adhesions. In addition, both total internal reflection fluorescence microscopy (TIRFM) and interference reflection microscopy (IRM) revealed a band of small punctate adhesions with rapid turnover near the cell leading margin. These changes led to an increase in global cell-substrate adhesion strength, as assessed by laminar flow experiments. Gleevec-treated cells have greater RhoA activity which, via myosin activation, led to an increase in the magnitude of total traction force applied to the substrate. These chemical and physical alterations upon Gleevec treatment produce the dramatic change in morphology and migration that is observed.
Note: Reproducció del document publicat a: 10.1371/journal.pone.0052233
It is part of: PLoS One, 2012, vol. 8, num. 1, p. e52233
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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