Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/44176
Title: A role for the tyrosine kinase ACK1 in neurotrophin signaling and neuronal extension and branching.
Author: La Torre i Vila, Anna
Masdeu, M.M.
Cotrufo, Tiziana
Moubarak, R. S.
Río Fernández, José Antonio del
Comella, J. X.
Soriano García, Eduardo
Ureña Bares, Jesús Mariano
Keywords: Sistema nerviós central
Interacció cel·lular
Transducció de senyal cel·lular
Proteïnes quinases
Central nervous system
Cell interaction
Cellular signal transduction
Protein kinases
Issue Date: 18-Apr-2013
Publisher: Nature Publishing Group
Abstract: Neurotrophins are involved in many crucial cellular functions, including neurite outgrowth, synapse formation, and plasticity. Although these events have long been known, the molecular determinants underlying neuritogenesis have not been fully characterized. Ack1 (activated Cdc42-associated tyrosine kinase) is a non-receptor tyrosine kinase that is highly expressed in the brain. Here, we demonstrate that Ack1 is a molecular constituent of neurotrophin signaling cascades in neurons and PC12 cells. We report that Ack1 interacts with Trk receptors and becomes tyrosine phosphorylated and its kinase activity is increased in response to neurotrophins. Moreover, our data indicate that Ack1 acts upstream of the Akt and MAPK pathways. We show that Ack1 overexpression induces neuritic outgrowth and promotes branching in neurotrophin-treated neuronal cells, whereas the expression of Ack1 dominant negatives or short-hairpin RNAs counteract neurotrophin-stimulated differentiation. Our results identify Ack1 as a novel regulator of neurotrophin-mediated events in primary neurons and in PC12 cells.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1038/cddis.2013.99
It is part of: Cell Death and Disease, 2013, vol. 4
Related resource: http://dx.doi.org/10.1038/cddis.2013.99
URI: http://hdl.handle.net/2445/44176
ISSN: 2041-4889
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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