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DC Field | Value | Language |
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dc.contributor.author | Llaudó Vallmajor, Inés | - |
dc.contributor.author | Cassis, L. | - |
dc.contributor.author | Torras Ambròs, Joan | - |
dc.contributor.author | Bestard Matamoros, Oriol | - |
dc.contributor.author | Franquesa, Marcella | - |
dc.contributor.author | Cruzado, Josep Ma. | - |
dc.contributor.author | Cerezo, Gema | - |
dc.contributor.author | Castaño Boldú, Esther | - |
dc.contributor.author | Pétriz, Jordi | - |
dc.contributor.author | Herrero Fresneda, Immaculada | - |
dc.contributor.author | Grinyó Boira, Josep M. | - |
dc.contributor.author | Lloberas Blanch, Núria | - |
dc.date.accessioned | 2013-09-17T09:58:24Z | - |
dc.date.available | 2013-09-17T09:58:24Z | - |
dc.date.issued | 2012-07-15 | - |
dc.identifier.issn | 1482-1826 | - |
dc.identifier.uri | http://hdl.handle.net/2445/46026 | - |
dc.description.abstract | Purpose. P-glycoprotein (Pgp) is a member of the ABC-transporter family that transports substances across cellular membranes acting as an efflux pump extruding drugs out of the cells. Pgp plays a key role on the pharmacokinetics of several dr ugs. Herein, we have studied the effects of immunosuppressants on Pgp function, assessing rhodamine-123 (Rho123) uptake and efflux in different T- cell subsets. Methods. Different immunosuppressants such as Cyclosporine (CsA), Rapamycin (Rapa) and Tacrolimus (Tac) were used to assess the in vitro effect on Pgp function of main T-cell subsets among healthy volunteers. We measured Rho123 upta ke, efflux and kinetic of extrusion in CD4 + and CD8 + subsets by flow cytometry. Antigen-specific memory T-ce ll responses were assessed by measuring T-cell proliferation and cytokine secretion using an allogeneic mixed lymphocyte reaction. Results. Rho123 uptake in groups treated with CsA and CsA+Rapa was signif icantly decreased compared to non-treated group and the other immunosupressants in both T cells subsets. Pgp activity was also reduced in CsA and CsA+Rapa compared to the other immunosupressants but it was only significant in the CsA group for CD8 + subset. Kinetic extrusion of Rho123 by Pgp in all groups was faster in CD8 + T cells. All immunosuppressants and the specific Pgp inhibitor PSC833 diminished antigen-primed T-cell proliferation, especially CD8 + T-cell subset. Conclusions. Our data indicate that small molecules immunosuppressants, especially CsA, inhibit Pgp activity and T-cell function being the CD8 + T cells more susceptible to this effect. These findings support the importance of Pgp when designing combined immunosuppressive regimens. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Canadian Society for Pharmaceutical Sciences | - |
dc.relation.isformatof | Reproducció del document publicat a: http://ejournals.library.ualberta.ca/index.php/JPPS/article/view/17188/14189 | - |
dc.relation.ispartof | Journal of Pharmacy and Pharmaceutical Sciences, 2012, vol. 15, num. 3, p. 407-419 | - |
dc.rights | cc-by-sa (c) Llaudó, I. et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Immunosupressió | - |
dc.subject.classification | Transport biològic | - |
dc.subject.classification | Proteïnes de membrana | - |
dc.subject.other | Immunosuppression | - |
dc.subject.other | Biological transport | - |
dc.subject.other | Membrane proteins | - |
dc.title | Impact of small molecules immunosuppressants on P-glycoprotein activity and T-cell function | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 621681 | - |
dc.date.updated | 2013-09-17T09:58:24Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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