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Title: Neurotoxicity of prion peptides mimicking the central domain of the cellular prion protein
Author: Vilches Saez, Silvia
Vergara Paños, Cristina
Nicolás i Pallejà, Josep Oriol
Sanclimens Pérez de Rozas, Glòria
Varón, S.
Acosta, Gerardo A.
Albericio Palomera, Fernando
Royo Expósito, Miriam
Río Fernández, José Antonio del
Gavín Marín, Rosalina
Keywords: Prions
Síntesi de pèptids
Mort cel·lular
Peptide synthesis
Cell death
Issue Date: 5-Aug-2013
Publisher: Public Library of Science (PLoS)
Abstract: The physiological functions of PrP(C) remain enigmatic, but the central domain, comprising highly conserved regions of the protein may play an important role. Indeed, a large number of studies indicate that synthetic peptides containing residues 106-126 (CR) located in the central domain (CD, 95-133) of PrP(C) are neurotoxic. The central domain comprises two chemically distinct subdomains, the charge cluster (CC, 95-110) and a hydrophobic region (HR, 112-133). The aim of the present study was to establish the individual cytotoxicity of CC, HR and CD. Our results show that only the CD peptide is neurotoxic. Biochemical, Transmission Electron Microscopy and Atomic Force Microscopy experiments demonstrated that the CD peptide is able to activate caspase-3 and disrupt the cell membrane, leading to cell death.
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It is part of: PLoS One, 2013, vol. 8, num. 8, p. e70881
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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