Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/47224
Title: Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
Author: Río Fernández, José Antonio del
Soriano García, Eduardo
Keywords: Neurobiologia
Regeneració del sistema nerviós
Hipocamp (Cervell)
Neurones
Neurobiology
Nervous system regeneration
Hippocampus (Brain)
Neurons
Issue Date: Jan-2009
Publisher: Nature Publishing Group
Abstract: We present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. The degree of axonal regeneration after treatment in lesioned cultures can be seen directly using green fluorescent protein (GFP) transgenic mice or by axon tracing and histological methods. Possible changes in cell morphology after pharmacological treatment can be determined easily by focal in vitro electroporation. The well-preserved cytoarchitectonics in the co-culture facilitate the analysis of identified cells or regenerating axons. The protocol takes up to a month.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1038/nprot.2009.202
It is part of: Nature Protocols, 2009, vol. 5, num. 2, p. 217-226
Related resource: http://dx.doi.org/10.1038/nprot.2009.202
URI: http://hdl.handle.net/2445/47224
ISSN: 1754-2189
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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