Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/48023
Title: Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
Author: Gresa Arribas, Núria
Viéitez, Cristina
Dentesano, Guido
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
Keywords: Micròglia
Neurotoxicologia
Malalties neurodegeneratives
Terapèutica
Microglia
Neurotoxicology
Neurodegenerative Diseases
Therapeutics
Issue Date: 20-Dec-2012
Publisher: Public Library of Science (PLoS)
Abstract: Neuron-microglia co-cultures treated with pro-inflammatory agents are a useful tool to study neuroinflammation in vitro, where to test the potential neuroprotective effect of anti-inflammatory compounds. However, a great diversity of experimental conditions can be found in the literature, making difficult to select the working conditions when considering this approach for the first time. We compared the use of neuron-primary microglia and neuron-BV2 cells (a microglial cell line) co-cultures, using different neuron:microglia ratios, treatments and time post-treatment to induce glial activation and derived neurotoxicity. We show that each model requires different experimental conditions, but that both neuron-BV2 and neuron-primary microglia LPS/IFN-γ-treated co-cultures are good to study the potential neuroprotective effect of anti-inflammatory agents. The contribution of different pro-inflammatory parameters in the neurotoxicity induced by reactive microglial cells was determined. IL-10 pre-treatment completely inhibited LPS/IFN-γ-induced TNF-α and IL-6 release, and COX-2 expression both in BV2 and primary microglial cultures, but not NO production and iNOS expression. However, LPS/IFN-γ induced neurotoxicity was not inhibited in IL-10 pre-treated co-cultures. The inhibition of NO production using the specific iNOS inhibitor 1400 W totally abolished the neurotoxic effect of LPS/IFN-γ, suggesting a major role for NO in the neurotoxic effect of activated microglia. Consequently, among the anti-inflammatory agents, special attention should be paid to compounds that inhibit NO production.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0045227
It is part of: PLoS One, 2012, vol. 7, num. 9, p. e45227
Related resource: http://dx.doi.org/10.1371/journal.pone.0045227
URI: http://hdl.handle.net/2445/48023
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

Files in This Item:
File Description SizeFormat 
617320.pdf2.12 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons