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Title: Lipid phosphate phosphatase 3 participates in transport carrier formation and protein trafficking in the early secretory pathway
Author: Gutiérrez-Martínez, Enric
Fernández Ulibarri, Inés
Lázaro Diéguez, Francisco
Johannes, Ludger
Pyne, Susan
Sarri Plans, Elisabet
Egea Guri, Gustavo
Keywords: Aparell de Golgi
Reticle endoplasmàtic
Golgi apparatus
Endoplasmic reticulum
Issue Date: 16-Apr-2013
Publisher: The Company of Biologists
Abstract: The inhibition of phosphatidic acid phosphatase (PAP) activity by propanolol indicates that diacylglycerol (DAG) is required for the formation of transport carriers at the Golgi and for retrograde trafficking to the ER. Here we report that the PAP2 family member lipid phosphate phosphatase 3 (LPP3, also known as PAP2b) localizes in compartments of the secretory pathway from ER export sites to the Golgi complex. The depletion of human LPP3: (i) reduces the number of tubules generated from the ER-Golgi intermediate compartment and the Golgi, with those formed from the Golgi being longer in LPP3-silenced cells than in control cells; (ii) impairs the Rab6-dependent retrograde transport of Shiga toxin subunit B from the Golgi to the ER, but not the anterograde transport of VSV-G or ssDsRed; and (iii) induces a high accumulation of Golgi-associated membrane buds. LPP3 depletion also reduces levels of de novo synthesized DAG and the Golgi-associated DAG contents. Remarkably, overexpression of a catalytically inactive form of LPP3 mimics the effects of LPP3 knockdown on Rab6-dependent retrograde transport. We conclude that LPP3 participates in the formation of retrograde transport carriers at the ER-Golgi interface, where it transitorily cycles, and during its route to the plasma membrane.
Note: Versió postprint del document publicat a:
It is part of: Journal of Cell Science, 2013, vol. 126, num. 12, p. 2641-2655
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ISSN: 0021-9533
Appears in Collections:Articles publicats en revistes (Biomedicina)

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