Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/48648
Title: Role of the bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes
Author: March, C.
Cano, V.
Moranta, D.
Llobet, Enrique
Pérez-Gutiérrez, C.
Tomàs Magaña, Juan
Suarez, T.
Garmendia, Junkal
Bengoechea, José Antonio
Keywords: Klebsiella pneumoniae
Bacteris
Klebsiella pneumoniae
Bacteria
Issue Date: Feb-2013
Publisher: Public Library of Science (PLoS)
Abstract: Phagocytosis is a key process of the immune system. The human pathogen Klebsiella pneumoniae is a well known example of a pathogen highly resistant to phagocytosis. A wealth of evidence demonstrates that the capsule polysaccharide (CPS) plays a crucial role in resistance to phagocytosis. The amoeba Dictyostelium discoideum shares with mammalian macrophages the ability to phagocytose and kill bacteria. The fact that K. pneumoniae is ubiquitous in nature and, therefore, should avoid predation by amoebae, poses the question whether K. pneumoniae employs similar means to counteract amoebae and mammalian phagocytes. Here we developed an assay to evaluate K. pneumoniae-D. discoideum interaction. The richness of the growth medium affected the threshold at which the cps mutant was permissive for Dictyostelium and only at lower nutrient concentrations the cps mutant was susceptible to predation by amoebae. Given the critical role of bacterial surface elements on host-pathogen interactions, we explored the possible contribution of the lipopolysaccharide (LPS) and outer membrane proteins (OMPs) to combat phagoyctosis by D. discoideum. We uncover that, in addition to the CPS, the LPS O-polysaccharide and the first core sugar participate in Klebsiella resistance to predation by D. discoideum. K. pneumoniae LPS lipid A decorations are also necessary to avoid predation by amoebae although PagP-dependent palmitoylation plays a more important role than the lipid A modification with aminoarabinose. Mutants lacking OMPs OmpA or OmpK36 were also permissive for D. discoideium growth. Except the LPS O-polysaccharide mutants, all mutants were more susceptible to phagocytosis by mouse alveolar macrophages. Finally, we found a correlation between virulence, using the pneumonia mouse model, and resistance to phagocytosis. Altogether, this work reveals novel K. pneumoniae determinants involved in resistance to phagocytosis and supports the notion that Dictyostelium amoebae might be useful as host model to measure K. pneumoniae virulence and not only phagocytosis.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0056847
It is part of: PLoS One, 2013, vol. 8, p. e56847
Related resource: http://dx.doi.org/10.1371/journal.pone.0056847
URI: http://hdl.handle.net/2445/48648
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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