Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/48844
Title: Effects of a cocoa diet on an intestinal inflammation model in rats
Author: Pérez Berezo, Teresa
Ramírez Santana, Carolina
Franch i Masferrer, Àngels
Ramos Romero, Sara
Castellote i Bargalló, M. Cristina
Pérez-Cano, Francisco J.
Castell, Margarida
Keywords: Cacau
Flavonoides
Colitis
Intestins
Dieta
Rates (Animals de laboratori)
Cocoa
Flavonoids
Colitis
Intestines
Diet
Rats as laboratory animals
Issue Date: 26-Oct-2012
Publisher: SAGE
Abstract: Cocoa is a rich source of fiber and flavonoids with recognized antioxidant and anti-inflammatory potential. The aim of this study was to evaluate the effects of a cocoa-enriched diet on rats with dextran sulphate sodium (DSS)-induced colitis. Wistar rats were fed with either a 5% cocoa diet or standard diet. Colon inflammation was induced by DSS in the drinking water: 5% for 6 days and 2% over the following 9 days. Colitis was assessed by body weight loss, stool consistency and blood presence in stools. A group of animals fed standard diet was treated with quercitrin (1 mg/kg) after colitis establishment. After 2 weeks of DSS treatment, the colon oxidative and inflammatory status and lymphocyte composition from blood and mesenteric lymph nodes (MLN) were assessed. The cocoa-fed group did not exhibit amelioration of clinical colitis but displayed higher antioxidant activity than the colitic reference group by the restoration of colon glutathione content and prevention of lipid peroxidation. The cocoa diet showed anti-inflammatory potential because it down-regulated serum TNF-alpha, colon iNOS activity and decreased colon cell infiltration. Lymphocyte composition in MLN was not modified by drinking DSS, but there was an increase in the proportion of NK and regulatory T cells in the blood. These changes were not modified by cocoa. In conclusion, cocoa intake may help to inhibit the negative oxidative effects consequent to colitis, although this action is not enough to abrogate the intestinal inflammation significantly.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1258/ebm.2012.012083
It is part of: Experimental Biology and Medicine, 2012, vol. 237, num. 10, p. 1181-1188
Related resource: http://dx.doi.org/10.1258/ebm.2012.012083
URI: http://hdl.handle.net/2445/48844
ISSN: 1535-3702
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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