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Title: G Protein-Coupled Receptor heterodimerization in the brain
Author: Borroto-Escuela, Dasiel O.
Romero Fernández, Wilber
Garriga, Pere
Ciruela Alférez, Francisco
Narváez, Manuel
Tarakanov, Alexander O.
Palkovits, Miklós
Agnati, Luigi F.
Fuxe, Kjell
Keywords: Cervell
Proteïnes G
G Proteins
Issue Date: 2013
Publisher: Elsevier
Abstract: G protein-coupled receptors (GPCRs) play critical roles in cellular processes and signaling and have been shown to form heteromers with diverge biochemical and/or pharmacological activities that are different from those of the corresponding monomers or homomers. However, despite extensive experimental results supporting the formation of GPCR heteromers in heterologous systems, the existence of such receptor heterocomplexes in the brain remains largely unknown, mostly because of the lack of appropriate methodology. Herein, we describe the in situ proximity ligation assay procedure underlining its high selectivity and sensitivity to image GPCR heteromers with confocal microscopy in brain sections. We describe here how the assay is performed and discuss advantages and disadvantages of this method compared with other available techniques.
Note: Versió postprint del document publicat a:
It is part of: Methods in Enzymology, 2013, vol. 521, p. 281-294
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ISSN: 0076-6879
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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