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Title: Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion.
Author: Isern, Joan
Martín-Antonio, Beatriz
Ghazanfari, Roshanak
Martín, Aan M.
López, Juan A.
Toro, Raquel del
Sánchez-Aguilera, Abel
Arranz, Lorena
Martín-Pérez, Daniel
Suárez-Lledó, María
Marín, Pedro
Van Pel, Melissa
Fibbe, Willem E.
Vázquez, Jesús
Scheding, Stefan
Urbano Ispizua, Álvaro
Méndez-Ferrer, Simón
Keywords: Diferenciació cel·lular
Medul·la òssia
Cell diferentiation
Bone marrow
Issue Date: 25-Apr-2013
Publisher: Elsevier
Abstract: Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs.
Note: Reproducció del document publicat a:
It is part of: Cell Reports, 2013, vol. 3, num. 5, p. 1714-1724
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ISSN: 2211-1247
Appears in Collections:Articles publicats en revistes (Medicina)

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