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|Title:||Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion.|
Martín, Aan M.
López, Juan A.
Toro, Raquel del
Van Pel, Melissa
Fibbe, Willem E.
Urbano Ispizua, Álvaro
|Abstract:||Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs.|
|Note:||Reproducció del document publicat a: http://dx.doi.org/10.1016/j.celrep.2013.03.041|
|It is part of:||Cell Reports, 2013, vol. 3, num. 5, p. 1714-1724|
|Appears in Collections:||Articles publicats en revistes (Medicina)|
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