Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/51643
Title: Effects of Cadmium and mercury on the upper part of skeletal muscle glycolysis in mice.
Author: Ramírez Bajo, María José
Atauri Carulla, Ramón de
Ortega Saine, Fernando
Westerhoff, Hans V.
Gelpí Buchaca, Josep Lluís
Centelles Serra, Josep Joan
Cascante i Serratosa, Marta
Keywords: Enzims
Mercuri
Cadmi
Glucòlisi
Aparell locomotor
Ratolins
Enzymes
Mercury
Cadmium
Glycolysis
Musculoskeletal system
Mice
Issue Date: 28-Jan-2014
Publisher: Public Library of Science (PLoS)
Abstract: The effects of pre-incubation with mercury (Hg2+) and cadmium (Cd2+) on the activities of individual glycolytic enzymes, on the flux and on internal metabolite concentrations of the upper part of glycolysis were investigated in mouse muscle extracts. In the range of metal concentrations analysed we found that only hexokinase and phosphofructokinase, the enzymes that shared the control of the flux, were inhibited by Hg2+ and Cd2+. The concentrations of the internal metabolites glucose-6-phosphate and fructose-6-phosphate did not change significantly when Hg2+ and Cd2+ were added. A mathematical model was constructed to explore the mechanisms of inhibition of Hg2+ and Cd2+ on hexokinase and phosphofructokinase. Equations derived from detailed mechanistic models for each inhibition were fitted to the experimental data. In a concentration-dependent manner these equations describe the observed inhibition of enzyme activity. Under the conditions analysed, the integral model showed that the simultaneous inhibition of hexokinase and phosphofructokinase explains the observation that the concentrations of glucose-6-phosphate and fructose-6-phosphate did not change as the heavy metals decreased the glycolytic flux.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0080018
It is part of: PLoS One, 2014, vol. 9, num. 1, p. e80018
Related resource: http://dx.doi.org/10.1371/journal.pone.0080018
URI: http://hdl.handle.net/2445/51643
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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