Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/52244
Title: The sea urchin metallothionein system: Comparative evaluation of the SpMTA and SpMTB metal-binding preferences.
Author: Tomas, Mireia
Domènech Casal, Jordi
Capdevila Vidal, Mercè
Bofill, Roger
Atrian i Ventura, Sílvia
Keywords: Eriçons de mar
Metal·loproteïnes
Metabolisme
Síntesi proteica
Cadmi
Zinc
Coure
Metalls pesants
Sea urchins
Metalloproteins
Metabolism
Protein synthesis
Cadmium
Zinc
Copper
Heavy metals
Issue Date: 2013
Publisher: Elsevier
Abstract: Metallothioneins (MTs) constitute a superfamily of ubiquitous metal-binding proteins of low molecular weight and high Cys content. They are involved in metal homeostasis and detoxification, amongst other proposed biological functions. Two MT isoforms (SpMTA and SpMTB) have been reported in the echinoderm Strongylocentrotus purpuratus (sea urchin), both containing 20 Cys residues and presenting extremely similar sequences, although showing distinct tissular and ontogenic expression patterns. Although exhaustive information is available for the Cd(II)-SpMTA complex, this including the full resolution of its 3D structure, no data has been reported concerning either SpMTA Zn(II) and Cu(I) binding properties, or the characterization of SpMTB at protein level. In this work, both the SpMTA and SpMTB isoforms, as well as their separate α and β domains, have been recombinantly synthesized in the presence of Zn(II), Cd(II) or Cu(II), and the corresponding metal complexes have been analyzed using electrospray mass spectrometry, and CD, ICP-AES and UV-vis spectroscopies. The results clearly show a better performance of isoform A when binding Zn(II) and Cd(II), and of isoform B when coordinating Cu(I). Thus, our results confirm the differential metal binding preference of SpMTA and SpMTB, which, together with the reported induction pattern of the respective genes, highlights how also in Echinodermata the MT polymorphism may be linked to the evolution of different physiological roles.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1016/j.fob.2013.01.005
It is part of: FEBS Open Bio, 2013, vol. 3, num. , p. 89-100
Related resource: http://dx.doi.org/10.1016/j.fob.2013.01.005
URI: http://hdl.handle.net/2445/52244
ISSN: 2211-5463
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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