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Title: An investigation of the resolution of inflammation (catabasis) in COPD.
Author: Noguera, Aina
Gomez, Cristina
Faner, Rosa
Cosío, Borja G.
González Périz, Ana
Clària i Enrich, Joan
Carvajal, Angel
Agustí García-Navarro, Àlvar
Keywords: Malalties pulmonars obstructives cròniques
Chronic obstructive pulmonary diseases
Issue Date: 13-Nov-2012
Publisher: BioMed Central
Abstract: Background: Chronic Obstructive Pulmonary Disease (COPD) is characterized by an enhanced inflammatory response to smoking that persists despite quitting. The resolution of inflammation (catabasis) is a complex and highly regulated process where tissue resident macrophages play a key role since they phagocytose apoptotic cells (efferocytosis), preventing their secondary necrosis and the spill-over of their pro-inflammatory cytoplasmic content, and release pro-resolution and tissue repair molecules, such as TGFβ, VEGF and HGF. Because inflammation does not resolve in COPD, we hypothesized that catabasis may be abnormal in these patients. Methods: To explore this hypothesis, we studied lung tissue samples obtained at surgery from 21 COPD patients,22 smokers with normal spirometry and 13 non-smokers controls. In these samples we used: (1)immunohistochemistry to assess the expression of CD44, CD36, VEGF and TGFβ in lung macrophages; (2) real time PCR to determine HGF, PPARγ, TGFβ, VEGF and MMP-9 gene expression; and, (3) ELISA to quantify lipoxin A4, a lipid mediator of catabasis. Results: We found that current and former smokers with COPD showed: (1) more inflammation (higher MMP-9 expression); (2) reduced macrophage surface expression of CD44, a key efferocytosis receptor; and, (3) similar levels of TGFβ, VEGF, HGF, PPARγ, and lipoxin A4 than smokers with normal spirometry, despite the presence of inflammation and disease. Conclusions: These results identify several potential abnormalities of catabasis in patients with COPD.
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It is part of: Respiratory Research, 2012, vol. 13, p. 101
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ISSN: 1465-9921
Appears in Collections:Articles publicats en revistes (Medicina)

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