Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/52724
Title: | An investigation of the resolution of inflammation (catabasis) in COPD. |
Author: | Noguera, Aina Gomez, Cristina Faner, Rosa Cosío, Borja G. González Périz, Ana Clària i Enrich, Joan Carvajal, Angel Agustí García-Navarro, Àlvar |
Keywords: | Malalties pulmonars obstructives cròniques Bronquitis Immunologia Chronic obstructive pulmonary diseases Bronchitis Immunology |
Issue Date: | 13-Nov-2012 |
Publisher: | BioMed Central |
Abstract: | Background: Chronic Obstructive Pulmonary Disease (COPD) is characterized by an enhanced inflammatory response to smoking that persists despite quitting. The resolution of inflammation (catabasis) is a complex and highly regulated process where tissue resident macrophages play a key role since they phagocytose apoptotic cells (efferocytosis), preventing their secondary necrosis and the spill-over of their pro-inflammatory cytoplasmic content, and release pro-resolution and tissue repair molecules, such as TGFβ, VEGF and HGF. Because inflammation does not resolve in COPD, we hypothesized that catabasis may be abnormal in these patients. Methods: To explore this hypothesis, we studied lung tissue samples obtained at surgery from 21 COPD patients,22 smokers with normal spirometry and 13 non-smokers controls. In these samples we used: (1)immunohistochemistry to assess the expression of CD44, CD36, VEGF and TGFβ in lung macrophages; (2) real time PCR to determine HGF, PPARγ, TGFβ, VEGF and MMP-9 gene expression; and, (3) ELISA to quantify lipoxin A4, a lipid mediator of catabasis. Results: We found that current and former smokers with COPD showed: (1) more inflammation (higher MMP-9 expression); (2) reduced macrophage surface expression of CD44, a key efferocytosis receptor; and, (3) similar levels of TGFβ, VEGF, HGF, PPARγ, and lipoxin A4 than smokers with normal spirometry, despite the presence of inflammation and disease. Conclusions: These results identify several potential abnormalities of catabasis in patients with COPD. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1186/1465-9921-13-101 |
It is part of: | Respiratory Research, 2012, vol. 13, p. 101 |
URI: | http://hdl.handle.net/2445/52724 |
Related resource: | http://dx.doi.org/10.1186/1465-9921-13-101 |
ISSN: | 1465-9921 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
625715.pdf | 1.22 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License