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DC Field | Value | Language |
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dc.contributor.author | Montori Grau, Marta | - |
dc.contributor.author | Guitart de la Rosa, Maria | - |
dc.contributor.author | García Martínez, Celia | - |
dc.contributor.author | Orozco, Anna | - |
dc.contributor.author | Gómez Foix, Anna Maria | - |
dc.date.accessioned | 2014-03-21T13:01:34Z | - |
dc.date.available | 2014-03-21T13:01:34Z | - |
dc.date.issued | 2011-11-04 | - |
dc.identifier.issn | 1471-2091 | - |
dc.identifier.uri | http://hdl.handle.net/2445/52754 | - |
dc.description.abstract | Background PPP1R6 is a protein phosphatase 1 glycogen-targeting subunit (PP1-GTS) abundant in skeletal muscle with an undefined metabolic control role. Here PPP1R6 effects on myotube glycogen metabolism, particle size and subcellular distribution are examined and compared with PPP1R3C/PTG and PPP1R3A/GM. Results PPP1R6 overexpression activates glycogen synthase (GS), reduces its phosphorylation at Ser-641/0 and increases the extracted and cytochemically-stained glycogen content, less than PTG but more than GM. PPP1R6 does not change glycogen phosphorylase activity. All tested PP1-GTS-cells have more glycogen particles than controls as found by electron microscopy of myotube sections. Glycogen particle size is distributed for all cell-types in a continuous range, but PPP1R6 forms smaller particles (mean diameter 14.4 nm) than PTG (36.9 nm) and GM (28.3 nm) or those in control cells (29.2 nm). Both PPP1R6- and GM-derived glycogen particles are in cytosol associated with cellular structures; PTG-derived glycogen is found in membrane- and organelle-devoid cytosolic glycogen-rich areas; and glycogen particles are dispersed in the cytosol in control cells. A tagged PPP1R6 protein at the C-terminus with EGFP shows a diffuse cytosol pattern in glucose-replete and -depleted cells and a punctuate pattern surrounding the nucleus in glucose-depleted cells, which colocates with RFP tagged with the Golgi targeting domain of β-1,4-galactosyltransferase, according to a computational prediction for PPP1R6 Golgi location. Conclusions PPP1R6 exerts a powerful glycogenic effect in cultured muscle cells, more than GM and less than PTG. PPP1R6 protein translocates from a Golgi to cytosolic location in response to glucose. The molecular size and subcellular location of myotube glycogen particles is determined by the PPP1R6, PTG and GM scaffolding. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/1471-2091-12-57 | - |
dc.relation.ispartof | BMC Biochemistry, 2011, vol. 12, num. 57 | - |
dc.relation.uri | http://dx.doi.org/10.1186/1471-2091-12-57 | - |
dc.rights | cc-by (c) Montori Grau, Marta et al., 2011 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Regulació del metabolisme | - |
dc.subject.classification | Regulació genètica | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.classification | Aparell locomotor | - |
dc.subject.classification | Metabolisme | - |
dc.subject.classification | Glicogen | - |
dc.subject.classification | Fetge | - |
dc.subject.classification | Àcids grassos | - |
dc.subject.other | Metabolic regulation | - |
dc.subject.other | Genetic regulation | - |
dc.subject.other | Gene expression | - |
dc.subject.other | Musculoskeletal system | - |
dc.subject.other | Metabolism | - |
dc.subject.other | Glycogen | - |
dc.subject.other | Liver | - |
dc.subject.other | Fatty acids | - |
dc.title | Differential pattern of glycogen accumulation after protein phosphatase 1 glycogen-targeting subunit PPP1R6 overexpression, compared to PPP1R3C and PPP1R3A, in skeletal muscle cells | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 606315 | - |
dc.date.updated | 2014-03-21T13:01:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22054094 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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606315.pdf | 1.13 MB | Adobe PDF | View/Open |
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