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Title: A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
Author: Blasco, Lucía
Veiga Crespo, Patricia
Viñas, Miquel
Villa, Tomás G.
Keywords: Fermentació
Saccharomyces cerevisiae
Saccharomyces cerevisiae
Issue Date: 1-Dec-2011
Publisher: Spanish Society for Microbiology (SEM) and Viguera Editores SL
Abstract: Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.
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It is part of: International Microbiology, 2011, vol. 14, p. 201-206
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ISSN: 1139-6709
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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