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Title: Elastic properties and secondary structure formation of single-stranded DNA at monovalent and divalent salt conditions
Author: Bosco, Alessandro
Camuñas Soler, Joan
Ritort Farran, Fèlix
Keywords: Reparació de l'ADN
Àcids nucleics
Aparells i instruments científics
DNA repair
Nucleic acids
Scientific apparatus and instruments
Issue Date: 12-Nov-2013
Publisher: Oxford University Press
Abstract: Single-stranded DNA (ssDNA) plays a major role in several biological processes. It is therefore of fundamental interest to understand how the elastic response and the formation of secondary structures are modulated by the interplay between base pairing and electrostatic interactions. Here we measure force-extension curves (FECs) of ssDNA molecules in optical tweezers set up over two orders of magnitude of monovalent and divalent salt conditions, and obtain its elastic parameters by fitting the FECs to semiflexible models of polymers. For both monovalent and divalent salts, we find that the electrostatic contribution to the persistence length is proportional to the Debye screening length, varying as the inverse of the square root of cation concentration. The intrinsic persistence length is equal to 0.7 nm for both types of salts, and the effectivity of divalent cations in screening electrostatic interactions appears to be 100-fold as compared with monovalent salt, in line with what has been recently reported for single-stranded RNA. Finally, we propose an analysis of the FECs using a model that accounts for the effective thickness of the filament at low salt condition and a simple phenomenological description that quantifies the formation of non-specific secondary structure at low forces.
Note: Reproducció del document publicat a:
It is part of: Nucleic Acids Research, 2013, vol. 42, num. 3, p. 2064-2074
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ISSN: 0305-1048
Appears in Collections:Articles publicats en revistes (Física de la Matèria Condensada)

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