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http://hdl.handle.net/2445/53665
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DC Field | Value | Language |
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dc.contributor.author | Mannara, F. | - |
dc.contributor.author | Valente, Tony | - |
dc.contributor.author | Saura Martí, Josep | - |
dc.contributor.author | Graus Ribas, Francesc | - |
dc.contributor.author | Saiz Hinarejos, Albert | - |
dc.contributor.author | Moreno, Beatriz | - |
dc.date.accessioned | 2014-04-28T11:46:02Z | - |
dc.date.available | 2014-04-28T11:46:02Z | - |
dc.date.issued | 2012-12-26 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/53665 | - |
dc.description.abstract | Experimental autoimmune encephalomyelitis (EAE) is the most relevant animal model to study demyelinating diseases such as multiple sclerosis. EAE can be induced by active (active EAE) or passive (at-EAE) transfer of activated T cells in several species and strains of rodents. However, histological features of at-EAE model in C57BL/6 are poorly described. The aim of this study was to characterize the neuroinflammatory and neurodegenerative responses of at-EAE in C57BL/6 mice by histological techniques and compare them with that observed in the active EAE model. To develop the at-EAE, splenocytes from active EAE female mice were harvested and cultured in presence of MOG 35-55 and IL-12, and then injected intraperitoneally in recipient female C57BL6/J mice. In both models, the development of EAE was similar except for starting before the onset of symptoms and presenting a higher EAE cumulative score in the at-EAE model. Spinal cord histological examination revealed an increased glial activation as well as more extensive demyelinating areas in the at-EAE than in the active EAE model. Although inflammatory infiltrates composed by macrophages and T lymphocytes were found in the spinal cord and brain of both models, B lymphocytes were significantly increased in the at-EAE model. The co-localization of these B cells with IgG and their predominant distribution in areas of demyelination would suggest that IgG-secreting B cells are involved in the neurodegenerative processes associated with at-EAE. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0052361 | - |
dc.relation.ispartof | PLoS One, 2012, vol. 7, p. e52361 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0052361 | - |
dc.rights | cc-by (c) Mannara et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Malalties neurodegeneratives | - |
dc.subject.classification | Cèl·lules B | - |
dc.subject.classification | Encefalomielitis | - |
dc.subject.other | Neurodegenerative Diseases | - |
dc.subject.other | B cells | - |
dc.subject.other | Encephalomyelitis | - |
dc.title | Passive experimental autoimmune encephalomyelitis in C57BL/6 with MOG: evidence of involvement of B cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 623620 | - |
dc.date.updated | 2014-04-28T11:46:02Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23300649 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
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File | Description | Size | Format | |
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623620.pdf | 1.11 MB | Adobe PDF | View/Open |
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