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http://hdl.handle.net/2445/53946
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DC Field | Value | Language |
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dc.contributor.author | Rebollo de Grado, Alba | - |
dc.contributor.author | Roglans i Ribas, Núria | - |
dc.contributor.author | Baena Muñoz, Miguel | - |
dc.contributor.author | Padrosa, Anna | - |
dc.contributor.author | Sánchez Peñarroya, Rosa M. | - |
dc.contributor.author | Merlos Roca, Manuel | - |
dc.contributor.author | Alegret i Jordà, Marta | - |
dc.contributor.author | Laguna Egea, Juan Carlos | - |
dc.date.accessioned | 2014-05-09T11:17:33Z | - |
dc.date.available | 2014-05-09T11:17:33Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.uri | http://hdl.handle.net/2445/53946 | - |
dc.description.abstract | High consumption of fructose-sweetened beverages has been linked to a high prevalence of chronic metabolic diseases. We have previously shown that a short course of fructose supplementation as a liquid solution induces glucose intolerance in female rats. In the present work, we characterized the fructose-driven changes in the liver and the molecular pathways involved. To this end, female rats were supplemented or not with liquid fructose (10%, w/v) for 7 or 14 days. Glucose and pyruvate tolerance tests were performed, and the expression of genes related to insulin signaling, gluconeogenesis and nutrient sensing pathways was evaluated. Fructose-supplemented rats showed increased plasma glucose excursions in glucose and pyruvate tolerance tests and reduced hepatic expression of several genes related to insulin signaling, including insulin receptor substrate 2 (IRS-2). However, the expression of key gluconeogenic enzymes, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase, was reduced. These effects were caused by an inactivation of hepatic forkhead box O1 (FoxO1) due to an increase in its acetylation state driven by a reduced expression and activity of sirtuin 1 (SIRT1). Further contributing to FoxO1 inactivation, fructose consumption elevated liver expression of the spliced form of X-box-binding-protein-1 as a consequence of an increase in the activity of the mammalian target of rapamycin 1 and protein 38-mitogen activated protein kinase (p38-MAPK). Liquid fructose affects both insulin signaling (IRS-2 and FoxO1) and nutrient sensing pathways (p38-MAPK, mTOR and SIRT1), thus disrupting hepatic insulin signaling without increasing the expression of key gluconeogenic enzymes. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.jnutbio.2013.10.014 | - |
dc.relation.ispartof | Journal of Nutritional Biochemistry, 2014, vol. 25, num. 2, p. 250-258 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.jnutbio.2013.10.014 | - |
dc.rights | (c) Elsevier B.V., 2014 | - |
dc.source | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) | - |
dc.subject.classification | Resistència a la insulina | - |
dc.subject.classification | Fetge | - |
dc.subject.classification | Fructosa | - |
dc.subject.other | Insulin resistance | - |
dc.subject.other | Liver | - |
dc.subject.other | Fructose | - |
dc.title | Liquid fructose down-regulates liver insulin receptor substrate 2 and gluconeogeneic enzymes by modifying nutrient sensing factors in rats | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 637133 | - |
dc.date.updated | 2014-05-09T11:17:33Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24445051 | - |
Appears in Collections: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
Files in This Item:
File | Description | Size | Format | |
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637133.pdf | 426.99 kB | Adobe PDF | View/Open |
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