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Title: Cholesterol regulates Syntaxin 6 trafficking at the TGN-endosomal boundaries
Author: Reverter Martín, Meritxell
Rentero Alfonso, Carles
Garcia-Melero, Ana
Hoque, Monira
Vilà de Muga, Sandra
Alvarez-Guaita, Anna
Conway, James
Wood, Peta
Cairns, Rose
Lykopoulou, Lila
Grinberg Vaisman, Daniel Raúl
Vilageliu i Arqués, Lluïsa
Bosch, Marta (Bosch Rodríguez)
Heeren, Joerg
Blasi Cabús, Joan
Timpson, Paul
Pol i Sorolla, Albert
Tebar Ramon, Francesc
Murray, Rachael Z.
Grewal, Thomas
Keywords: Colesterol
Transport biològic
Biologia molecular
Aparell de Golgi
Biological transport
Molecular biology
Golgi apparatus
Issue Date: 8-May-2014
Publisher: Elsevier
Abstract: Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.
Note: Reproducció del document publicat a:
It is part of: Cell Reports, 2014, vol. 7, num. 3, p. 883-897
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ISSN: 2211-1247
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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