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Title: Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains.
Author: Kassan, Adam
Herms, Albert
Fernández-Vidal, Andrea
Bosch, Marta (Bosch Rodríguez)
Schieber, Nicole L.
Reddy, Babu J. N.
Fajardo, Alba
Gelabert Baldrich, Mariona
Tebar Ramon, Francesc
Enrich Bastús, Carles
Gross, Steven P.
Parton, Robert G.
Pol i Sorolla, Albert
Keywords: Coenzims
Metabolisme dels lípids
Transport biològic
Lipid metabolism
Biological transport
Issue Date: 23-Dec-2013
Publisher: Rockefeller University Press
Abstract: Control of lipid droplet (LD) nucleation and copy number are critical, yet poorly understood, processes. We use model peptides that shift from the endoplasmic reticulum (ER) to LDs in response to fatty acids to characterize the initial steps of LD formation occurring in lipid-starved cells. Initially, arriving lipids are rapidly packed in LDs that are resistant to starvation (pre-LDs). Pre-LDs are restricted ER microdomains with a stable core of neutral lipids. Subsequently, a first round of"emerging" LDs is nucleated, providing additional lipid storage capacity. Finally, in proportion to lipid concentration, new rounds of LDs progressively assemble. Confocal microscopy and electron tomography suggest that emerging LDs are nucleated in a limited number of ER microdomains after a synchronized stepwise process of protein gathering, lipid packaging, and recognition by Plin3 and Plin2. A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly sites, where it is required for efficient LD nucleation and lipid storage.
Note: Reproducció del document publicat a:
It is part of: Journal of Cell Biology, 2013, vol. 203, num. 6, p. 985-1001
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ISSN: 0021-9525
Appears in Collections:Articles publicats en revistes (Biomedicina)

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