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Title: Bacterial membrane injuries induced by lactacin F and nisin
Author: Dalmau, M.
Maier, Elke
Mulet, Natalia
Viñas, Miquel
Benz, Roland
Keywords: Pèptids
Membranes cel·lulars
Citometria de fluxe
Cell membranes
Flow cytometry
Issue Date: Jun-2002
Publisher: Spanish Society for Microbiology (SEM) and Viguera Editores SL
Abstract: The combined action of nisin and lactacin F, two bacteriocins produced by lactic acid bacteria, is additive. In this report, the basis of this effect is examined. Channels formed by lactacin F were studied by experiments using planar lipid bilayers, and bactericidal effects were analyzed by flow cytometry. Lactacin F produced pores with a conductance of 1 ns in black lipid bilayers in 1 mM KClat 10 mV at 20°C. Pore formation was strongly dependent on voltage. Although lactacin F formed pores at very low potential (10 mV), the dependence was exponentialabov e 40 mV. The injuries induced by nisin and lactacin F in the membranes of Lactobacillus helveticus produced different flow cytometric profiles. Probably, when both bacteriocins are present, each acts separately; their cooperation may be due to an increase in the number of single membrane injuries
Note: Reproducció del document publicat a:
It is part of: International Microbiology, 2002, vol. 5, num. 2, p. 73-80
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ISSN: 1139-6709
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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