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Title: Pint lincRNA connects the p53 pathway with epigenetic silencing by the Polycomb repressive complex 2
Author: Marin-Bejar, Oskar
Marchese, Francesco P.
Athie, Alejandro
Sanchez, Yolanda
Gonzalez, Jovanna
Segura, Victor
Huang, Lulu
Moreno, Isabel
Navarro Ponz, Alfons
Monzó Planella, Mariano
Garcia-Foncillas, Jesús
Rinn, John L.
Guo, Shuling
Huarte, Maite
Keywords: Transcripció genètica
Regulació genètica
Genetic transcription
Genetic regulation
Issue Date: 26-Sep-2013
Publisher: BioMed Central
Abstract: BACKGROUND: The p53 transcription factor is located at the core of a complex wiring of signaling pathways that are critical for the preservation of cellular homeostasis. Only recently it has become clear that p53 regulates the expression of several long intergenic noncoding RNAs (lincRNAs). However, relatively little is known about the role that lincRNAs play in this pathway. RESULTS: Here we characterize a lincRNA named Pint (p53 induced noncoding transcript). We show that Pint is a ubiquitously expressed lincRNA that is finely regulated by p53. In mouse cells, Pint promotes cell proliferation and survival by regulating the expression of genes of the TGF-β, MAPK and p53 pathways. Pint is a nuclear lincRNA that directly interacts with the Polycomb repressive complex 2 (PRC2), and is required for PRC2 targeting of specific genes for H3K27 tri-methylation and repression. Furthermore, Pint functional activity is highly dependent on PRC2 expression. We have also identified Pint human ortholog (PINT), which presents suggestive analogies with the murine lincRNA. PINT is similarly regulated by p53, and its expression significantly correlates with the same cellular pathways as the mouse ortholog, including the p53 pathway. Interestingly, PINT is downregulated in colon primary tumors, while its overexpression inhibits the proliferation of tumor cells, suggesting a possible role as tumor suppressor. CONCLUSIONS: Our results reveal a p53 autoregulatory negative mechanism where a lincRNA connects p53 activation with epigenetic silencing by PRC2. Additionally, we show analogies and differences between the murine and human orthologs, identifying a novel tumor suppressor candidate lincRNA.
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It is part of: Genome Biology, 2013, vol. 14, num. 9, p. R104
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ISSN: 1474-760X
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

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