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Title: Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection
Author: Puigdellívol Sánchez, Anna
Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
Keywords: Transport biològic
Nervis espinals
Rates (Animals de laboratori)
Biological transport
Spinal nerves
Rats as laboratory animals
Issue Date: 15-Dec-1998
Publisher: Elsevier B.V.
Abstract: The present study was designed to investigate the efficacy of the fluorescent dyes Fast Blue (FB), Fluoro-Gold (FG), and Diamidino Yellow (DY) for retrograde tracing of lumbar dorsal root ganglia after their subcutaneous injection into different hindlimb digits. Injection of equal volumes (0.5 mu l) of 5% FB or 2% FG resulted in similar mean numbers of sensory neurones labelled by each tracer. Injection of equal volumes (0.5 mu l) of FB or FG in a single digit followed 10 days later by a second injection of the same volume of 5% DY into the same digit resulted in similar mean numbers of labelled sensory neurones for each of the three tracers. Furthermore, on average, 75% of all the FB-labelled cells and 74% of all FC-labelled cells also contained DY. Repeating the same experiment with an increased volume of DY (1.5 mu l) resulted in an increase in the mean number of double-labelled profiles to 82 and 84% for FB and FG, respectively. The results show that FB, FG and DY label similar numbers of cutaneous afferents and that a high level of double labelling may be obtained after sequential injections in digits. These properties make them suitable candidates in investigations where a combination of tracers with similar labelling efficacies is needed.
Note: Versió postprint del document publicat a:
It is part of: Journal of Neuroscience Methods, 1998, vol. 86, num. 1, p. 7-16
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ISSN: 0165-0270
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

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