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Title: | Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection |
Author: | Puigdellívol Sánchez, Anna Prats Galino, Alberto Ruano Gil, Domingo Molander, Carl |
Keywords: | Transport biològic Nervis espinals Pell Rates (Animals de laboratori) Biological transport Spinal nerves Skin Rats as laboratory animals |
Issue Date: | 15-Dec-1998 |
Publisher: | Elsevier B.V. |
Abstract: | The present study was designed to investigate the efficacy of the fluorescent dyes Fast Blue (FB), Fluoro-Gold (FG), and Diamidino Yellow (DY) for retrograde tracing of lumbar dorsal root ganglia after their subcutaneous injection into different hindlimb digits. Injection of equal volumes (0.5 mu l) of 5% FB or 2% FG resulted in similar mean numbers of sensory neurones labelled by each tracer. Injection of equal volumes (0.5 mu l) of FB or FG in a single digit followed 10 days later by a second injection of the same volume of 5% DY into the same digit resulted in similar mean numbers of labelled sensory neurones for each of the three tracers. Furthermore, on average, 75% of all the FB-labelled cells and 74% of all FC-labelled cells also contained DY. Repeating the same experiment with an increased volume of DY (1.5 mu l) resulted in an increase in the mean number of double-labelled profiles to 82 and 84% for FB and FG, respectively. The results show that FB, FG and DY label similar numbers of cutaneous afferents and that a high level of double labelling may be obtained after sequential injections in digits. These properties make them suitable candidates in investigations where a combination of tracers with similar labelling efficacies is needed. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(98)00137-X |
It is part of: | Journal of Neuroscience Methods, 1998, vol. 86, num. 1, p. 7-16 |
URI: | http://hdl.handle.net/2445/56744 |
Related resource: | http://dx.doi.org/10.1016/S0165-0270(98)00137-X |
ISSN: | 0165-0270 |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
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