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DC Field | Value | Language |
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dc.contributor.author | Puigdellívol Sánchez, Anna | - |
dc.contributor.author | Prats Galino, Alberto | - |
dc.contributor.author | Ruano Gil, Domingo | - |
dc.contributor.author | Molander, Carl | - |
dc.date.accessioned | 2014-09-05T09:51:42Z | - |
dc.date.available | 2014-09-05T09:51:42Z | - |
dc.date.issued | 1998-12-15 | - |
dc.identifier.issn | 0165-0270 | - |
dc.identifier.uri | http://hdl.handle.net/2445/56744 | - |
dc.description.abstract | The present study was designed to investigate the efficacy of the fluorescent dyes Fast Blue (FB), Fluoro-Gold (FG), and Diamidino Yellow (DY) for retrograde tracing of lumbar dorsal root ganglia after their subcutaneous injection into different hindlimb digits. Injection of equal volumes (0.5 mu l) of 5% FB or 2% FG resulted in similar mean numbers of sensory neurones labelled by each tracer. Injection of equal volumes (0.5 mu l) of FB or FG in a single digit followed 10 days later by a second injection of the same volume of 5% DY into the same digit resulted in similar mean numbers of labelled sensory neurones for each of the three tracers. Furthermore, on average, 75% of all the FB-labelled cells and 74% of all FC-labelled cells also contained DY. Repeating the same experiment with an increased volume of DY (1.5 mu l) resulted in an increase in the mean number of double-labelled profiles to 82 and 84% for FB and FG, respectively. The results show that FB, FG and DY label similar numbers of cutaneous afferents and that a high level of double labelling may be obtained after sequential injections in digits. These properties make them suitable candidates in investigations where a combination of tracers with similar labelling efficacies is needed. | - |
dc.format.extent | 23 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(98)00137-X | - |
dc.relation.ispartof | Journal of Neuroscience Methods, 1998, vol. 86, num. 1, p. 7-16 | - |
dc.relation.uri | http://dx.doi.org/10.1016/S0165-0270(98)00137-X | - |
dc.rights | (c) Elsevier B.V., 1998 | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Transport biològic | - |
dc.subject.classification | Nervis espinals | - |
dc.subject.classification | Pell | - |
dc.subject.classification | Rates (Animals de laboratori) | - |
dc.subject.other | Biological transport | - |
dc.subject.other | Spinal nerves | - |
dc.subject.other | Skin | - |
dc.subject.other | Rats as laboratory animals | - |
dc.title | Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 502145 | - |
dc.date.updated | 2014-09-05T09:51:42Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 9894781 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
Files in This Item:
File | Description | Size | Format | |
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502145.pdf | 360.48 kB | Adobe PDF | View/Open |
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