Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/59231
Title: Fatty acid transport protein 1 (FATP1) delivered into skeletal muscle localizes in mitochondria and regulates lipid and ketone body disposal
Author: Guitart de la Rosa, Maria
Osorio-Conles, O.
Pentinat Pelegrin, Thais
Cebrià, J.
García-Villoria, J.
Sala Cano, David
Sebastián Muñoz, David
Zorzano Olarte, Antonio
Ribes Rubió, Antònia
Jiménez Chillarón, José Carlos
García Martínez, Celia
Gómez Foix, Anna Maria
Keywords: Aparell locomotor
Metabolisme energètic
Glicogen
Àcids grassos
Diabetis
Proteïnes de membrana
Mitocondris
Musculoskeletal system
Energy metabolism
Glycogen
Fatty acids
Diabetes
Membrane proteins
Mitochondria
Issue Date: 23-May-2014
Publisher: Public Library of Science (PLoS)
Abstract: FATP1 mediates skeletal muscle cell fatty acid import, yet its intracellular localization and metabolic control role are not completely defined. Here, we examine FATP1 localization and metabolic effects of its overexpression in mouse skeletal muscle. The FATP1 protein was detected in mitochondrial and plasma membrane fractions, obtained by differential centrifugation, of mouse gastrocnemius muscle. FATP1 was most abundant in purified mitochondria, and in the outer membrane and soluble intermembrane, but not in the inner membrane plus matrix, enriched subfractions of purified mitochondria. Immunogold electron microscopy localized FATP1-GFP in mitochondria of transfected C2C12 myotubes. FATP1 was overexpressed in gastrocnemius mouse muscle, by adenovirus-mediated delivery of the gene into hindlimb muscles of newborn mice, fed after weaning a chow or high-fat diet. Compared to GFP delivery, FATP1 did not alter body weight, serum fed glucose, insulin and triglyceride levels, and whole-body glucose tolerance, in either diet. However, fatty acid levels were lower and beta-hydroxybutyrate levels were higher in FATP1-than GFP-mice, irrespective of diet. Moreover, intramuscular triglyceride content was lower in FATP1-versus GFP-mice regardless of diet, and beta-hydroxybutyrate content was unchanged in high-fat-fed mice. Electroporation-mediated FATP1 overexpression enhanced palmitate oxidation to CO2, but not to acid-soluble intermediate metabolites, while CO2 production from beta-hydroxybutyrate was inhibited and that from glucose unchanged, in isolated mouse gastrocnemius strips. In summary, FATP1 was localized in mitochondria, in the outer membrane and intermembrane parts, of mouse skeletal muscle, what may be crucial for its metabolic effects. Overexpressed FATP1 enhanced disposal of both systemic fatty acids and intramuscular triglycerides. Consistently, it did not contribute to the high-fat diet-induced metabolic dysregulation. However, FATP1 lead to hyperketonemia, likely secondary to the sparing of ketone body oxidation by the enhanced oxidation of fatty acids.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0098109
It is part of: PLoS One, 2014, vol. 9, num. 5, p. e98109
Related resource: http://dx.doi.org/10.1371/journal.pone.0098109
URI: http://hdl.handle.net/2445/59231
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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