Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/60796
Title: SMRT-mediated co-shuttling enables export of class IIa HDACs independent of their CaM kinase phosphorylation sites
Author: Soriano Zaragoza, Francesc X. (Francesc Xavier)
Chawla, S.
Skehel, P.
Hardingham, G.E.
Keywords: Neurobiologia
Cicle cel·lular
Proteïnes
Enzims
Malalties neurodegeneratives
Neurobiology
Cell cycle
Proteins
Enzymes
Neurodegenerative Diseases
Issue Date: 1-Jan-2013
Publisher: Wiley
Abstract: The Class IIa histone deacetylases (HDAC)4 and HDAC5 play a role in neuronal survival and behavioral adaptation in the CNS. Phosphorylation at 2/3 N-terminal sites promote their nuclear export. We investigated whether non-canonical signaling routes to Class IIa HDAC export exist because of their association with the co-repressor Silencing Mediator Of Retinoic And Thyroid Hormone Receptors (SMRT). We found that, while HDAC5 and HDAC4 mutants lacking their N-terminal phosphorylation sites (HDAC4(MUT), HDAC5(MUT)) are constitutively nuclear, co-expression with SMRT renders them exportable by signals that trigger SMRT export, such as synaptic activity, HDAC inhibition, and Brain Derived Neurotrophic Factor (BDNF) signaling. We found that SMRT's repression domain 3 (RD3) is critical for co-shuttling of HDAC5(MUT), consistent with the role for this domain in Class IIa HDAC association. In the context of BDNF signaling, we found that HDAC5(WT), which was more cytoplasmic than HDAC5(MUT), accumulated in the nucleus after BDNF treatment. However, co-expression of SMRT blocked BDNF-induced HDAC5(WT) import in a RD3-dependent manner. In effect, SMRT-mediated HDAC5(WT) export was opposing the BDNF-induced HDAC5 nuclear accumulation observed in SMRT's absence. Thus, SMRT's presence may render Class IIa HDACs exportable by a wider range of signals than those which simply
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1111/jnc.12058
It is part of: Journal of Neurochemistry, 2013, vol. 124, num. 1, p. 26-35
Related resource: http://dx.doi.org/10.1111/jnc.12058
URI: http://hdl.handle.net/2445/60796
ISSN: 0022-3042
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Files in This Item:
File Description SizeFormat 
624824.pdf748.21 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.