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Title: Functional Identification of Proteus mirabilis eptC gene encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase
Author: Aquilini, Eleonora
Merino Montero, Susana
Knirel, Yuriy A.
Regué Queralt, Miguel, 1953-
Tomàs Magaña, Juan
Keywords: Bacteris patògens
Genètica bacteriana
Pathogenic bacteria
Bacterial genetics
Issue Date: 21-Apr-2014
Publisher: MDPI
Abstract: By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
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It is part of: International Journal of Molecular Sciences, 2014, vol. 15, p. 6689-6702
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ISSN: 1422-0067
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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