Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/61471
Title: Aeromonas surface glucan attached throught the O-antigen ligase represents a new way to obtain UDP-Glucose
Author: Merino Montero, Susana
Bouamama, Lamiaa
Knirel, Yuriy A.
Shenchenkova, Sofya N.
Regué Queralt, Miguel, 1953-
Tomàs Magaña, Juan
Keywords: Bacteris
Glucosa
Polisacàrids
Enzims
Reacció en cadena de la polimerasa
Bacteria
Glucose
Polysaccharides
Enzymes
Polymerase chain reaction
Issue Date: 1-May-2012
Publisher: Public Library of Science (PLoS)
Abstract: We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan).
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0035707
It is part of: PLoS One, 2012, vol. 7, num. 5, p. e35707
Related resource: http://dx.doi.org/10.1371/journal.pone.0035707
URI: http://hdl.handle.net/2445/61471
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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