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Title: | Aeromonas surface glucan attached throught the O-antigen ligase represents a new way to obtain UDP-Glucose |
Author: | Merino Montero, Susana Bouamama, Lamiaa Knirel, Yuriy A. Shenchenkova, Sofya N. Regué Queralt, Miguel, 1953- Tomàs Magaña, Juan |
Keywords: | Bacteris Glucosa Polisacàrids Enzims Reacció en cadena de la polimerasa Bacteria Glucose Polysaccharides Enzymes Polymerase chain reaction |
Issue Date: | 1-May-2012 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan). |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0035707 |
It is part of: | PLoS One, 2012, vol. 7, num. 5, p. e35707 |
URI: | http://hdl.handle.net/2445/61471 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0035707 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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