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Title: | A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil |
Author: | Padilha, Itácio Q. M. Valenzuela Mayorga, Susana Valeria Grisi, Teresa Díaz Lucea, Pilar de Araújo, Demetrius Pastor Blasco, Francisco I. Javier |
Keywords: | Bacils Ecologia microbiana Enzims microbians Biopolímers Sòls Bacillus (Bacteria) Microbial ecology Microbial enzymes Biopolymers Soils |
Issue Date: | 2014 |
Publisher: | Spanish Society for Microbiology (SEM) and Viguera Editores SL |
Abstract: | A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg<br>1, a Km of 4.0 mg/ml, and a kcat of 13,333 min<br>1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184] |
Note: | Reproducció del document publicat a: http://dx.doi.org/:10.2436/20.1501.01.220. |
It is part of: | International Microbiology, 2014, vol. 17, num. 3, p. 175-184 |
URI: | http://hdl.handle.net/2445/61481 |
Related resource: | http://dx.doi.org/10.2436/20.1501.01.220. |
ISSN: | 1139-6709 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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