Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/61481
Title: A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil
Author: Padilha, I.Q.M.
Valenzuela Mayorga, Susana Valeria
Grisi, T.C.S.L.
Díaz Lucea, Pilar
Araújo, Demetrius A.M. de
Pastor Blasco, Francisco I. Javier
Keywords: Bacils
Ecologia microbiana
Enzims microbians
Biopolímers
Sòls
Bacillus (Bacteria)
Microbial ecology
Microbial enzymes
Biopolymers
Soils
Issue Date: 2014
Publisher: Spanish Society for Microbiology (SEM) and Viguera Editores SL
Abstract: A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg<br>1, a Km of 4.0 mg/ml, and a kcat of 13,333 min<br>1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184]
Note: Reproducció del document publicat a: http://dx.doi.org/:10.2436/20.1501.01.220.
It is part of: International Microbiology, 2014, vol. 17, num. 3, p. 175-184
Related resource: http://dx.doi.org/10.2436/20.1501.01.220.
URI: http://hdl.handle.net/2445/61481
ISSN: 1139-6709
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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