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Title: GLUT2-mediated glucose uptake and availability are required for embryonic brain development in zebrafish
Author: Marín Juez, Rubén
Rovira, Mireia
Crespo Morao, Diego
Van der Vaart, M.
Spaink, H.P.
Planas Vilarnau, Josep
Keywords: Glucosa
Transport biològic
Peix zebra
Neurobiologia del desenvolupament
Biological transport
Zebra danio
Developmental neurobiology
Issue Date: Jan-2015
Publisher: International Society for Cerebral Blood Flow and Metabolism
Abstract: Glucose transporter 2 (GLUT2; gene name SLC2A2) has a key role in the regulation of glucose dynamics in organs central to metabolism. Although GLUT2 has been studied in the context of its participation in peripheral and central glucose sensing, its role in the brain is not well understood. To decipher the role of GLUT2 in brain development, we knocked down slc2a2 (glut2), the functional ortholog of human GLUT2, in zebrafish. Abrogation of glut2 led to defective brain organogenesis, reduced glucose uptake and increased programmed cell death in the brain. Coinciding with the observed localization of glut2 expression in the zebrafish hindbrain, glut2 deficiency affected the development of neural progenitor cells expressing the proneural genes atoh1b and ptf1a but not those expressing neurod. Specificity of the morphant phenotype was demonstrated by the restoration of brain organogenesis, whole-embryo glucose uptake, brain apoptosis, and expression of proneural markers in rescue experiments. These results indicate that glut2 has an essential role during brain development by facilitating the uptake and availability of glucose and support the involvement of glut2 in brain glucose sensing.
Note: Versió postprint del document publicat a:
It is part of: Journal of Cerebral Blood Flow and Metabolism, 2015, vol. 35, num. 1, p. 74-85
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ISSN: 0271-678X
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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