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http://hdl.handle.net/2445/62364
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DC Field | Value | Language |
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dc.contributor.author | Sainz Polo, M. A. | - |
dc.contributor.author | González Navarro, Beatriz | - |
dc.contributor.author | Pastor Blasco, Francisco I. Javier | - |
dc.contributor.author | Sanz Aparicio, J. | - |
dc.date.accessioned | 2015-02-04T15:27:25Z | - |
dc.date.available | 2015-02-04T15:27:25Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://hdl.handle.net/2445/62364 | - |
dc.description.abstract | A construct containing the CBM22-1-CBM22-2 tandem forming the N-terminal domain of Paenibacillus barcinonensis xylanase 10C (Xyn10C) has been purified and crystallized. A xylan-binding function and an affinity for mixed [beta]-1,3/[beta]-1,4 glucans have previously been demonstrated for some members of the CBM22 family. The sequence of the tandem is homologous to the N-terminal domains found in several thermophilic enzymes. Crystals of this tandem were grown by the streak-seeding method after a long optimization strategy. The structure has been determined by molecular replacement to a resolution of 2.43 Å and refinement is under way. This study represents the first structure containing two contiguous CBM22 modules, which will contribute to a better understanding of the role that this multiplicity plays in fine-tuning substrate affinity | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | International Union of Crystallography | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1107/S2053230X14027496 | - |
dc.relation.ispartof | Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2015, vol. 71, num. 2, p. 136-140 | - |
dc.relation.uri | http://dx.doi.org/10.1107/S2053230X14027496 | - |
dc.rights | (c) International Union of Crystallography, 2015 | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Enzims microbians | - |
dc.subject.classification | Biopolímers | - |
dc.subject.classification | Biotecnologia | - |
dc.subject.other | Microbial enzymes | - |
dc.subject.other | Biopolymers | - |
dc.subject.other | Biotechnology | - |
dc.title | Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1-CBM22-2 tandem | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 646327 | - |
dc.date.updated | 2015-02-04T15:27:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 25664784 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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646327.pdf | 817.62 kB | Adobe PDF | View/Open |
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