Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/63186
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Espinosa Parrilla, Juan Francisco | - |
dc.contributor.author | Martínez Moreno, Martín | - |
dc.contributor.author | Gasull Casanova, Xavier | - |
dc.contributor.author | Mahy Gehenne, Josette Nicole | - |
dc.contributor.author | Rodríguez Allué, Manuel José | - |
dc.date.accessioned | 2015-02-20T08:46:05Z | - |
dc.date.available | 2015-02-20T08:46:05Z | - |
dc.date.issued | 2015-12-12 | - |
dc.identifier.issn | 1044-7431 | - |
dc.identifier.uri | http://hdl.handle.net/2445/63186 | - |
dc.description.abstract | Under pathological conditions, microglia, the resident CNS immune cells, become reactive and release pro-inflammatory cytokines and neurotoxic factors. We investigated whether this phenotypic switch includes changes in the expression of the L-type voltage-gated calcium channel (VGCC) in a rat model of N-methyl-d-aspartate-induced hippocampal neurodegeneration. Double immunohistochemistry and confocal microscopy evidenced that activated microglia express the L-type VGCC. We then analyzed whether BV2 microglia express functional L-type VGCC, and investigated the latter's role in microglial cytokine release and phagocytic capacity. Activated BV2 microglia express the CaV1.2 and CaV1.3 subunits of the L-type VGCC determined by reverse transcription-polymerase chain reaction, Western blot and immunocytochemistry. Depolarization with KCl induced a Ca2+ entry facilitated by Bay k8644 and partially blocked with nifedipine, which also reduced TNF-α and NO release by 40%. However, no nifedipine effect on BV2 microglia viability or phagocytic capacity was observed. Our results suggest that in CNS inflammatory processes, the L-type VGCC plays a specific role in the control of microglial secretory activity. | - |
dc.format.extent | 39 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.mcn.2014.12.004 | - |
dc.relation.ispartof | Molecular and Cellular Neuroscience, 2015, vol. 64, p. 104-115 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.mcn.2014.12.004 | - |
dc.rights | (c) Elsevier B.V., 2015 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Micròglia | - |
dc.subject.classification | Canals de calci | - |
dc.subject.classification | Sistema nerviós central | - |
dc.subject.classification | Malalties neurodegeneratives | - |
dc.subject.other | Microglia | - |
dc.subject.other | Calcium channels | - |
dc.subject.other | Central nervous system | - |
dc.subject.other | Neurodegenerative Diseases | - |
dc.title | The L-type voltage-gated calcium channel modulates microglial pro-inflammatory activity | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 645255 | - |
dc.date.updated | 2015-02-20T08:46:10Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 25497271 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
645255.pdf | 851.69 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.