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Title: Combined dielectrophoretic and impedance system for on-chip controlled bacteria concentration: Application to Escherichia coli
Author: Moral, Beatriz del
Punter Villagrasa, Jaime
Oliva Brañas, Ana María
Álvarez Azpeitia, Juan Manuel
Colomer i Farrarons, Jordi
Samitier i Martí, Josep
Homs Corbera, Antoni
Miribel-Català, Pere Ll. (Pere Lluís)
Keywords: Escheríchia coli
Escherichia coli
Issue Date: 27-Apr-2015
Publisher: Wiley-VCH
Abstract: The present paper reports a bacteria autonomous controlled concentrator prototype with a user-friendly interface for bench-top applications. It is based on a micro-fluidic lab-on-a-chip and its associated custom instrumentation, which consists in a dielectrophoretic actuator, to pre-concentrate the sample, and an impedance analyser, to measure concentrated bacteria levels. The system is composed by a single micro-fluidic chamber with interdigitated electrodes and a instrumentation with custom electronics. The prototype is supported by a real-time platform connected to a remote computer, which automatically controls the system and displays impedance data used to monitor the status of bacteria accumulation on-chip. The system automates the whole concentrating operation. Performance has been studied for controlled volumes of Escherichia coli (E. coli) samples injected into the micro-fluidic chip at constant flow rate of 10 μL/min. A media conductivity correcting protocol has been developed, as the preliminary results showed distortion of the impedance analyser measurement produced by bacterial media conductivity variations through time. With the correcting protocol, the measured impedance values were related to the quantity of bacteria concentrated with a correlation of 0.988 and a coefficient of variation of 3.1%. Feasibility of E. coli on-chip automated concentration, using the miniaturized system, has been demonstrated. Furthermore, the impedance monitoring protocol had been adjusted and optimized, to handle changes in the electrical properties of the bacteria media over time.
Note: Versió postprint del document publicat a:
It is part of: Electrophoresis, 2015, vol. 36, num. 9-10, p. 1130-1141
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ISSN: 0173-0835
Appears in Collections:Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Electrònica)

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