Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/66250
Title: Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones
Author: López, Elena
Domènech, Arnau
Ferrándiz, María José
Frias, Maria Joao
Ardanuy Tisaire, María Carmen
Ramirez, Mario
García, Ernesto
Liñares Louzao, Josefina
Campa, Adela G. de la
Keywords: Pneumococs
Estreptococs
Resistència als medicaments
Malalties de l'aparell respiratori
Malalties cròniques
Streptococcus pneumonia
Streptococcus
Drug resistance
Respiratory diseases
Chronic diseases
Issue Date: 9-Apr-2014
Publisher: Public Library of Science (PLoS)
Abstract: Antibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone resistance occurs mainly by alteration of their intracellular targets, the type II DNA topoisomerases, which is acquired either by point mutation or by recombination. Increase in fluoroquinolone-resistance may depend on the balance between antibiotic consumption and the cost that resistance imposes to bacterial fitness. In addition, pneumococcal prophages could play an important role. Prophage induction by fluoroquinolones was confirmed in 4 clinical isolates by using Southern blot hybridization. Clinical isolates (105 fluoroquinolone-resistant and 160 fluoroquinolone-susceptible) were tested for lysogeny by using a PCR assay and functional prophage carriage was studied by mitomycin C induction. Fluoroquinolone-resistant strains harbored fewer inducible prophages (17/43) than fluoroquinolone-susceptible strains (49/70) (P = 0.0018). In addition, isolates of clones associated with fluoroquinolone resistance [CC156 (3/25); CC63 (2/20), and CC81 (1/19)], had lower frequency of functional prophages than isolates of clones with low incidence of fluoroquinolone resistance [CC30 (4/21), CC230 (5/20), CC62 (9/21), and CC180 (21/30)]. Likewise, persistent strains from patients with chronic respiratory diseases subjected to fluoroquinolone treatment had a low frequency of inducible prophages (1/11). Development of ciprofloxacin resistance was tested with two isogenic strains, one lysogenic and the other non-lysogenic: emergence of resistance was only observed in the non-lysogenic strain. These results are compatible with the lysis of lysogenic isolates receiving fluoroquinolones before the development of resistance and explain the inverse relation between presence of inducible prophages and fluoroquinolone-resistance.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0094358
It is part of: PLoS One, 2014, vol. 9, num. 4, p. e94358
Related resource: http://dx.doi.org/10.1371/journal.pone.0094358
URI: http://hdl.handle.net/2445/66250
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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