Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/66361
Title: Preparation of reference materials for anti-doping control: study of the lyophilisation conditions.
Other Titles: Preparación de materiales de referencia en el control anti-dopaje: estudio de las condiciones de liofilización.
Author: Gutiérrez Escolano, Ester
Director: Sahuquillo Estrugo, Àngels
Ventura Alemany, Rosa
Bergés Casas, Rosa
Keywords: Liofilització
Orina
Drogues
Tesis
Freeze-drying
Urine
Drugs of abuse
Theses
Issue Date: Jun-2015
Abstract: Urine reference materials used in interlaboratory comparison must be homogeneous and stable over time. The lyophilisation process is one of the methods applied when preparing urine reference materials for ensuring stability during distribution, transport and storage. Urine samples of 11- nor- Δ9- tetrahydrocannabinol- 9- carboxylic acid (THC-COOH), Benzoylecgonine (BE), Ethyl glucuronide/Ethyl sulphate (EtG/EtS) and ethanol (EtOH) were prepared from blank urine previously filtered. Samples were fortified with the adequate standard solutions and they were divided in aliquots. Aliquots stored at 4 ºC and lyophilized aliquots were used to evaluate the homogeneity of the samples. Others were frozen at -20 ºC and they were used as reference aliquots. In the first analysis, all prepared samples, except EtOH sample, were considered homogeneous. Concentration differences of THC-COOH and BE between lyophilized aliquots and reference aliquots were minimum (lower than 5 %). However, differences higher than 8 % were observed for EtG/EtS. If these differences were maintained constant in subsequence quantifications, they could be attributed to the evaporation of the analytes during the lyophilisation process. Results obtained for EtOH sample show a completely loss of the analyte due to its high volatility.
Note: Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2015, Tutors: Àngels Sahuquillo Estrugo, Rosa Ventura Alemany i Rosa Bergés Casas
URI: http://hdl.handle.net/2445/66361
Appears in Collections:Treballs Finals de Grau (TFG) - Química

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