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Title: Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
Author: Álvarez Palomo, Ana Belén
McLenachan, Samuel
Requena, Jordi
Menchón Najas, Cristina
Barrot i Feixat, Carme
Chen, F.
Munné Bosch, Sergi
Edel, Michael John
Keywords: Hormones vegetals
Cèl·lules mare embrionàries
Ratolins (Animals de laboratori)
Plant hormones
Embryonic stem cells
Mice (Laboratory animals)
Issue Date: 7-Jan-2014
Publisher: Mary Ann Liebert, Inc.
Abstract: Reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined pluripotency and self-renewal factors has taken stem cell technology to the forefront of regenerative medicine. However, a number of challenges remain in the field including efficient protocols and the threat of cancer. Reprogramming of plant somatic cells to plant embryonic stem cells using a combination of two plant hormones was discovered in 1957 and has been a routine university laboratory practical for over 30 years. The plant hormones responsible for cell reprogramming to pluripotency, indole-3-acetic acid (IAA) and isopentenyl adenosine (IPA), are present in human cells, leading to the exciting possibility that plant hormones might reprogram mammalian cells without genetic factors. We found that plant hormones on their own could not reprogram mammalian cells but increase the efficiency of the early formation of iPS cells combined with three defined genetic factors during the first 3 weeks of reprogramming by accelerating the cell cycle and regulating pluripotency genes. Moreover, the cytokinin IPA, a known human anticancer agent, reduced the threat of cancer of iPS cell in vitro by regulating key cancer and stem cell-related genes, most notably c-Myc and Igf-1. In conclusion, the plant hormones, auxin and cytokinin, are new small chemicals useful for enhancing early reprogramming efficiency of mammalian cells and reducing the threat of cancer from iPS cells. These findings suggest a novel role for plant hormones in the biology of mammalian cell plasticity
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It is part of: Stem Cells and Development, 2014, vol. 23, num. 6, p. 586-593
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ISSN: 1547-3287
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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