Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/66705
Title: Hha has a defined regulatory role that is not dependent upon H-NS or StpA
Author: Solórzano, Carla
Srikumar, Shabarinath
Canals, Rocío
Juárez Giménez, Antonio
Paytubi Casabona, Sònia
Madrid Xufré, Cristina
Keywords: Proteïnes
Salmonel·la
Genòmica
Proteins
Salmonella
Genomics
Issue Date: 30-Jul-2015
Publisher: Frontiers Media
Abstract: The Hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with H-NS-like proteins. Whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. Residue D48 of Hha protein is essential for the interaction with H-NS, thus the D48N substitution in Hha protein abrogates H-NS/Hha interaction. Despite being a paralog of H-NS protein, StpA interacts with HhaD48N with higher affinity than with the wild type Hha protein. To analyze whether Hha is capable of acting independently of H-NS and StpA, we conducted transcriptomic analysis on the hha and stpA deletion strains and the hhaD48N substitution strain of Salmonella Typhimurium using a custom microarray. The results obtained allowed the identification of 120 genes regulated by Hha in an H-NS/StpA-independent manner, 38% of which are horizontally acquired genes. A significant number of the identified genes are involved in functions related to cell motility, iron uptake, and pathogenicity. Thus, motility assays, siderophore detection and intra-macrophage replication assays were performed to confirm the transcriptomic data. Our findings point out the importance of Hha protein as an independent regulator in S. Typhimurium, highlighting a regulatory role on virulence.
Note: Reproducció del document publicat a: http://dx.doi.org/10.3389/fmicb.2015.00773
It is part of: Frontiers in Microbiology, 2015, vol. 6, p. 773-773
Related resource: http://dx.doi.org/10.3389/fmicb.2015.00773
URI: http://hdl.handle.net/2445/66705
ISSN: 1664-302X
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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