Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/67779
Title: In silico/in vivo insights into the functional and evolutionary pathway of Pseudomonas aeruginosa oleate-diol synthase. Discovery of a new bacterial di-heme cytochrome C peroxidase subfamily
Author: Estupiñan Romero, Mónica
Álvarez García, Daniel
Barril Alonso, Xavier
Díaz Lucea, Pilar
Manresa Presas, Ma. Ángeles (María Ángeles)
Keywords: Peroxidasa
Metal·loenzims
Pseudomonas
Seqüència d'aminoàcids
Peroxidase
Metalloenzymes
Pseudomonas
Amino acid sequence
Issue Date: 8-Jul-2015
Publisher: Public Library of Science (PLoS)
Abstract: As previously reported, P. aeruginosa genes PA2077 and PA2078 code for 10S-DOX (10S-Dioxygenase) and 7,10-DS (7,10-Diol Synthase) enzymes involved in long-chain fatty acid oxygenation through the recently described oleate-diol synthase pathway. Analysis of the amino acid sequence of both enzymes revealed the presence of two heme-binding motifs (CXXCH) on each protein. Phylogenetic analysis showed the relation of both proteins to bacterial di-heme cytochrome c peroxidases (Ccps), similar to Xanthomonas sp. 35Y rubber oxidase RoxA. Structural homology modelling of PA2077 and PA2078 was achieved using RoxA (pdb 4b2n) as a template. From the 3D model obtained, presence of significant amino acid variations in the predicted heme-environment was found. Moreover, the presence of palindromic repeats located in enzyme-coding regions, acting as protein evolution elements, is reported here for the first time in P. aeruginosa genome. These observations and the constructed phylogenetic tree of the two proteins, allow the proposal of an evolutionary pathway for P. aeruginosa oleate-diol synthase operon. Taking together the in silico and in vivo results obtained we conclude that enzymes PA2077 and PA2078 are the first described members of a new subfamily of bacterial peroxidases, designated as Fatty acid-di-heme Cytochrome c peroxidases (FadCcp).
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0131462
It is part of: PLoS One, 2015, vol. 10, num. 7, p. e0131462
Related resource: http://dx.doi.org/10.1371/journal.pone.0131462
URI: http://hdl.handle.net/2445/67779
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Files in This Item:
File Description SizeFormat 
653753.pdf5.59 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons