Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/68434
Title: Polar glycosylated and lateral non-glycosylated flagella from Aeromonas hydrophila strain AH-1 (serotype O11)
Author: Fulton, Kelly M.
Mendonza Barberá, Elena de
Twine, Susan M.
Tomàs Magaña, Juan
Merino Montero, Susana
Keywords: Glicoproteïnes
Bacteriòfags
Glycoproteins
Bacteriophages
Issue Date: 27-Nov-2015
Publisher: MDPI
Abstract: Polar and but not lateral flagellin proteins from Aeromonas hydrophila strain AH-1 (serotype O11) were found to be glycosylated. Top-down mass spectrometry studies of purified polar flagellins suggested the presence of a 403 Da glycan of mass. Bottom-up mass spectrometry studies showed the polar flagellin peptides to be modified with 403 Da glycans in O-linkage. The MS fragmentation pattern of this putative glycan was similar to that of pseudaminic acid derivative. Mutants lacking the biosynthesis of pseudaminic acid (pseB and pseI homologues) were unable to produce polar flagella but no changes were observed in lateral flagella by post transcriptional regulation of the flagellin. Complementation was achieved by reintroduction of the wild type pseB and pseI. We compared two pathogenic features (adhesion to eukaryotic cells and biofilm production) between the wild type strain and two kinds of mutants: mutants lacking polar flagella glycosylation and lacking the O11-antigen lipopolysaccharide (LPS) but with unaltered polar flagella glycosylation. Results suggest that polar flagella glycosylation is extremely important for A. hydrophila AH-1 adhesion to Hep-2 cells and biofilm formation. In addition, we show the importance of the polar flagella glycosylation for immune stimulation of IL-8 production via toll 'like' receptor 5 TLR5.
Note: Reproducció del document publicat a: http://dx.doi.org/10.3390/ijms161226097
It is part of: International Journal of Molecular Sciences, 2015, vol. 16, p. 28255-28269
Related resource: http://dx.doi.org/10.3390/ijms161226097
URI: http://hdl.handle.net/2445/68434
ISSN: 1422-0067
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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