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DC Field | Value | Language |
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dc.contributor.author | Alonso, Nuria | - |
dc.contributor.author | Julián, María Teresa | - |
dc.contributor.author | Carrascal, Jorge | - |
dc.contributor.author | Colobran, Roger | - |
dc.contributor.author | Pujol-Autonell, Irma | - |
dc.contributor.author | Teniente, Aina | - |
dc.contributor.author | Fernández, Marco Antonio | - |
dc.contributor.author | Miñarro Alonso, Antonio | - |
dc.contributor.author | María Ruiz de Villa, Carmen | - |
dc.contributor.author | Vives-Pi, Marta | - |
dc.contributor.author | Puig Domingo, Manuel | - |
dc.contributor.author | Rodriguez-Fernández, Silvia | - |
dc.date.accessioned | 2015-12-21T14:26:29Z | - |
dc.date.available | 2015-12-21T14:26:29Z | - |
dc.date.issued | 2015-11-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/68549 | - |
dc.description.abstract | CD26 is a T cell activation marker consisting in a type II transmembrane glycoprotein with dipeptidyl peptidase IV (DPPIV) activity in its extracellular domain. It has been described that DPPIV inhibition delays the onset of type 1 diabetes and reverses the disease in non-obese diabetic (NOD) mice. The aim of the present study was to assess the effect of MK626, a DPPIV inhibitor, in type 1 diabetes incidence and in T lymphocyte subsets at central and peripheral compartments. Pre-diabetic NOD mice were treated with MK626. Diabetes incidence, insulitis score, and phenotyping of T lymphocytes in the thymus, spleen and pancreatic lymph nodes were determined after 4 and 6 weeks of treatment, as well as alterations in the expression of genes encoding β-cell autoantigens in the islets. The effect of MK626 was also assessed in two in vitro assays to determine proliferative and immunosuppressive effects. Results show that MK626 treatment reduces type 1 diabetes incidence and after 6 weeks of treatment reduces insulitis. No differences were observed in the percentage of T lymphocyte subsets from central and peripheral compartments between treated and control mice. MK626 increased the expression of CD26 in CD8+ T effector memory (TEM) from spleen and pancreatic lymph nodes and in CD8+ T cells from islet infiltration. CD8+TEM cells showed an increased proliferation rate and cytokine secretion in the presence of MK626. Moreover, the combination of CD8+ TEM cells and MK626 induces an immunosuppressive response. In conclusion, treatment with the DPPIV inhibitor MK626 prevents experimental type 1 diabetes in association to increase expression of CD26 in the CD8+ TEM lymphocyte subset. In vitro assays suggest an immunoregulatory role of CD8+ TEM cells that may be involved in the protection against autoimmunity to β pancreatic islets associated to DPPIV inhibitor treatment. | - |
dc.format.extent | 22 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0142186 | - |
dc.relation.ispartof | PLoS One, 2015, vol. 10, num. 11, p. e0142186-e0142186 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0142186 | - |
dc.rights | cc-by (c) Alonso, Nuria et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Diabetis | - |
dc.subject.classification | Limfòcits | - |
dc.subject.classification | Citologia | - |
dc.subject.classification | Ratolins (Animals de laboratori) | - |
dc.subject.other | Diabetes | - |
dc.subject.other | Lymphocytes | - |
dc.subject.other | Cytology | - |
dc.subject.other | Mice (Laboratory animals) | - |
dc.title | Type1 Diabetes prevention in NOD mice by targeting DPPIV/CD26 is associated with changes in CD8+T effector memory subset | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 655904 | - |
dc.date.updated | 2015-12-21T14:26:29Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26555789 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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655904.pdf | 1.06 MB | Adobe PDF | View/Open |
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