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Title: Pathological Methods Applied to the Investigation of Causes of Death in Developing Countries: Minimally Invasive Autopsy Approach
Author: Castillo, Paola
Ussene, Esperança
Ismail, Mamudo R.
Jordão, Dercio
Lovane, Lucilia
Carrilho, Carla
Lorenzoni, Cesaltina
Lacerda, Marcus V. G.
Palhares, Antonio
Rodríguez-Carunchio, Leonardo
Martínez Yoldi, Miguel Julián
Vila Estapé, Jordi
Bassat Orellana, Quique
Menéndez, Clara
Ordi i Majà, Jaume
Keywords: Autòpsia
Causes de la mort
Salut pública
Humors corporals
Causes of death
Public health
Body fluids
Issue Date: 30-Jun-2015
Publisher: Public Library of Science (PLoS)
Abstract: BACKGROUND AND AIMS: Complete diagnostic autopsies (CDA) remain the gold standard in the determination of cause of death (CoD). However, performing CDAs in developing countries is challenging due to limited facilities and human resources, and poor acceptability. We aimed to develop and test a simplified minimally invasive autopsy (MIA) procedure involving organ-directed sampling with microbiology and pathology analyses implementable by trained technicians in low- income settings. METHODS: A standardized scheme for the MIA has been developed and tested in a series of 30 autopsies performed at the Maputo Central Hospital, Mozambique. The procedure involves the collection of 20 mL of blood and cerebrospinal fluid (CSF) and puncture of liver, lungs, heart, spleen, kidneys, bone marrow and brain in all cases plus uterus in women of childbearing age, using biopsy needles. RESULTS: The sampling success ranged from 67% for the kidney to 100% for blood, CSF, lung, liver and brain. The amount of tissue obtained in the procedure varied from less than 10 mm2 for the lung, spleen and kidney, to over 35 mm2 for the liver and brain. A CoD was identified in the histological and/or the microbiological analysis in 83% of the MIAs. CONCLUSIONS: A simplified MIA technique allows obtaining adequate material from body fluids and major organs leading to accurate diagnoses. This procedure could improve the determination of CoD in developing countries
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2015, vol. 10, num. 6, p. e0132057
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Fonaments Clínics)

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