Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/69246
Title: Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
Author: Mongui, A.
Pérez-Llanos, F. J.
Yamamoto, M. M.
Lozano Molero, Miguel
Zambrano, M.M.
Portillo Obando, Hernando A. del
Fernández Becerra, Carmen
Restrepo, S.
Junca, H.
Keywords: Genòmica
Genètica microbiana
Malària
Plasmodium falciparum
Resistència als medicaments
Efecte dels medicaments sobre els microorganismes
Genomics
Microbial genetics
Malaria
Plasmodium falciparum
Drug resistance
Effect of drugs on microorganisms
Issue Date: 4-Jun-2015
Publisher: BioMed Central
Abstract: BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-015-0748-6
It is part of: Malaria Journal, 2015, vol. 14, num. 233, p. 1-6
Related resource: http://dx.doi.org/10.1186/s12936-015-0748-6
URI: http://hdl.handle.net/2445/69246
ISSN: 1475-2875
Appears in Collections:Articles publicats en revistes (ISGlobal)

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