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Title: Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
Author: Mongui, Alvaro
Pérez-Llanos, Francy J.
Yamamoto, Marcio M.
Lozano, Marcela
Zambrano, Maria M.
Portillo, Patricis Del
Fernández Becerra, Carmen
Restrepo, Silvia
Junca, Howard
Director: Del Portillo, Hernando A.
Keywords: Genòmica
Genètica microbiana
Plasmodium falciparum
Resistència als medicaments
Efecte dels medicaments sobre els microorganismes
Microbial genetics
Plasmodium falciparum
Drug resistance
Effect of drugs on microorganisms
Issue Date: 4-Jun-2015
Publisher: BioMed Central
Abstract: BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed.
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It is part of: Malaria Journal, 2015, vol. 14, num. 233, p. 1-6
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ISSN: 1475-2875
Appears in Collections:Articles publicats en revistes (ISGlobal)

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