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http://hdl.handle.net/2445/69246
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DC Field | Value | Language |
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dc.contributor.advisor | Del Portillo, Hernando A. | - |
dc.contributor.author | Mongui, Alvaro | - |
dc.contributor.author | Pérez-Llanos, Francy J. | - |
dc.contributor.author | Yamamoto, Marcio M. | - |
dc.contributor.author | Lozano, Marcela | - |
dc.contributor.author | Zambrano, Maria M. | - |
dc.contributor.author | Portillo, Patricis Del | - |
dc.contributor.author | Fernández Becerra, María del Carmen | - |
dc.contributor.author | Restrepo, Silvia | - |
dc.contributor.author | Junca, Howard | - |
dc.date.accessioned | 2016-02-04T13:03:18Z | - |
dc.date.available | 2016-02-04T13:03:18Z | - |
dc.date.issued | 2015-06-04 | - |
dc.identifier.issn | 1475-2875 | - |
dc.identifier.uri | http://hdl.handle.net/2445/69246 | - |
dc.description.abstract | BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-015-0748-6 | - |
dc.relation.ispartof | Malaria Journal, 2015, vol. 14, num. 233, p. 1-6 | - |
dc.relation.uri | http://dx.doi.org/10.1186/s12936-015-0748-6 | - |
dc.rights | cc by (c) Mongui et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Genòmica | - |
dc.subject.classification | Genètica microbiana | - |
dc.subject.classification | Malària | - |
dc.subject.classification | Plasmodium falciparum | - |
dc.subject.classification | Resistència als medicaments | - |
dc.subject.classification | Efecte dels medicaments sobre els microorganismes | - |
dc.subject.other | Genomics | - |
dc.subject.other | Microbial genetics | - |
dc.subject.other | Malaria | - |
dc.subject.other | Plasmodium falciparum | - |
dc.subject.other | Drug resistance | - |
dc.subject.other | Effect of drugs on microorganisms | - |
dc.title | Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2016-02-02T15:34:04Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/245226/EU//MAGICPAH | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26040274 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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mongui2015_1899.pdf | 665.29 kB | Adobe PDF | View/Open |
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