Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/69282
Title: RNA stem-loop to G-quadruplex equilibrium controls mature miRNA production inside the cell
Author: Pandey, Satyaprakash
Agarwala, Prachi
Jayaraj, Gopal G.
Gargallo Gómez, Raimundo
Maiti, Souvik
Keywords: RNA
Porfirines
RNA
Porphyrins
Issue Date: 10-Nov-2015
Publisher: American Chemical Society
Abstract: Biological role for existence of overlapping structures in RNA is possible yet remains very less explored. G-rich tracts of RNA form G-quadruplexes while GC-rich sequences prefer stem-loop structures. Equilibrium between alternate structures within RNA may occur and influence its functionality. We tested equilibrium between G-quadruplex and stem-loop structure in RNA and its effect on biological processes using pre-miRNA as a model system. Dicer enzyme recognizes canonical stem-loop structures in pre-miRNA to produce mature miRNAs. Deviation from stem-loop leads to deregulated mature miRNA levels, providing readout of existence of alternate structure per se G-quadruplex mediated structural interference in miRNA maturation. In vitro analysis using beacon and Dicer cleavage assays indicated that mature miRNA levels depend on relative amounts of K+ and Mg2+ ions suggesting an ion-dependent structural shift. Further in cellulo studies with and without TmPyP4 (RNA G-quadruplex destabilizer) demonstrated that miRNA biogenesis is modulated by G-quadruplex-stem-loop equilibrium in a subset of pre-miRNAs. Our combined analysis thus provides evidence for formation of non-canonical G-quadruplexes in competition with canonical stem-loop structure inside the cell and its effect on miRNA maturation in a comprehensive manner.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1021/acs.biochem.5b00574
It is part of: Biochemistry, 2015, vol. 54, num. 48, p. 7067-7078
Related resource: http://dx.doi.org/10.1021/acs.biochem.5b00574
URI: http://hdl.handle.net/2445/69282
ISSN: 0006-2960
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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