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Title: Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: a useful approach for developing countries
Author: Selva Jové, Laura
Benmessaoud, Rachid
Lanaspa, Miguel
Jroundi, Imane
Moraleda, Cinta
Acácio, Sozinho
Iñigo, Melania
Bastiani, Alien
Monsonis, Manuel
Pallarés Giner, Roman
Bassat Orellana, Quique
Muñoz-Almagro, Carmen
Keywords: Bacteris patògens
Reacció en cadena de la polimerasa
Pathogenic bacteria
Polymerase chain reaction
Streptococcus pneumonia
Issue Date: 8-Oct-2013
Publisher: Public Library of Science (PLoS)
Abstract: Background: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001). Conclusion: Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2013, vol. 8, num. 10, p. e76970
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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